Genetic Background and Mobility of Variants of the Gene nleA in Attaching and Effacing Escherichia coli

DC FieldValueLanguage
dc.contributor.authorCreuzburg, Kristina
dc.contributor.authorHeeren, Sabine
dc.contributor.authorLis, Claudia M.
dc.contributor.authorKranz, Markus
dc.contributor.authorHensel, Michael
dc.contributor.authorSchmidt, Herbert
dc.date.accessioned2021-12-23T16:08:05Z-
dc.date.available2021-12-23T16:08:05Z-
dc.date.issued2011
dc.identifier.issn00992240
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/8226-
dc.description.abstractIn this study, we characterized the genetic background of various nleA variants in 106 Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) strains. The flanking regions of eight nleA variants were analyzed by DNA sequencing and compared with the corresponding regions of five previously described NleA-encoding prophages. The analyzed nleA variants were all located downstream of the DNA region responsible for phage morphogenesis. In particular, the type III effector genes avrA, ospB, nleH, and nleG and IS elements were detected in the neighborhood of nleA. The structure of the eight analyzed regions flanking nleA primarily resembled the corresponding region of the NleA(4795)-encoding prophage BP-4795. Using PCR, the gene order flanking 13 nleA variants in strains of different serogroups was compared to the respective regions in reference strains. The analyses showed that strains which harbor prophages with conserved flanking regions of a particular nleA variant predominantly occurred, and IS elements were additionally detected in these regions. We were able to mobilize nleA by transduction in 20% of strains determined, which comprised in particular EPEC strains harboring an nleA variant, the gene encoding the protein known as ``EspI-like.'' Plaque hybridization was used to identify phages that harbor the genes stx and nleA. However, only two strains harbored variant nleA(4795) in the genome of an Stx1 prophage.
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG) [Schm 1360/3-1]; This work was supported by grant Schm 1360/3-1 from the Deutsche Forschungsgemeinschaft (DFG).
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
dc.subjectBiotechnology & Applied Microbiology
dc.subjectCOMPLETE GENOME SEQUENCE
dc.subjectEFFECTOR
dc.subjectEVOLUTION
dc.subjectIDENTIFICATION
dc.subjectMicrobiology
dc.subjectO157
dc.subjectPROTEIN
dc.subjectSECRETION
dc.subjectVIRULENCE FACTOR NLEA
dc.titleGenetic Background and Mobility of Variants of the Gene nleA in Attaching and Effacing Escherichia coli
dc.typejournal article
dc.identifier.doi10.1128/AEM.06492-11
dc.identifier.isiISI:000298157800029
dc.description.volume77
dc.description.issue24
dc.description.startpage8705
dc.description.endpage8713
dc.contributor.orcid0000-0001-6046-4198
dc.identifier.eissn10985336
dc.publisher.place1752 N ST NW, WASHINGTON, DC 20036-2904 USA
dcterms.isPartOf.abbreviationAppl. Environ. Microbiol.
dcterms.oaStatusBronze, Green Published
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-6604-6253-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidHeMi480-
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