Applicability and robustness of the hen's egg test for analysis of micronucleus induction (HET-MN): Results from an inter-laboratory trial

Autor(en): Greywe, Daniela
Kreutz, Juergen
Banduhn, Norbert
Krauledat, Matthias
Scheel, Julia
Schroeder, Klaus R.
Wolf, Thorsten
Reisinger, Kerstin
Stichwörter: Alternatives to animal testing; Biotechnology & Applied Microbiology; BONE-MARROW; CHICK-EMBRYO; CODED CHEMICALS; DISCRIMINATE RODENT CARCINOGENS; Genetics & Heredity; Genotoxicity; Hen's egg; HUMAN-LYMPHOCYTES; IN-VITRO; Micronucleus test; MOUSE LYMPHOMA CELL; MUTAGENESIS ASSAY; Mutagenicity; SISTER-CHROMATID EXCHANGE; Toxicology; VITRO GENOTOXICITY TESTS
Erscheinungsdatum: 2012
Volumen: 747
Ausgabe: 1
Startseite: 118
Seitenende: 134
The hen's egg test for analysis of micronucleus formation (HET-MN) was developed several years ago to provide an alternative test system to the in vivo micronucleus test. In order to assess its applicability and robustness, a study was carried out at the University of Osnabrueck (lab A) and at the laboratories of Henkel AG & Co. KGaA (lab B). Following transfer of the method to lab B, a range of test substances that had been pre-tested at lab A, were tested at Henkel: the genotoxins cyclophosphamide, dimethylbenz(a)anthracene, methotrexate, acrylamide, azorubin, N-nitroso-dimethylamine and the non-genotoxins, orange G and isopropyl myristate. In a second phase, additional compounds with known in vivo properties were examined in both labs: the non-genotoxin, ampicillin, the ``irrelevant'' positives, isophorone and 2,4-dichlorophenol (''irrelevant'' means positive in standard in vitro tests, but negative in vivo), the clastogen p-chloroaniline, and the aneugens carbendazim and vinorelbine. All substances were correctly predicted in both labs with respect to their in vivo genotoxic properties, indicating that the HET-MN may have an improved predictivity compared with current standard in vitro test systems. The results support the promising role of the HET-MN assay as a supplement to existing test batteries. (C) 2012 Elsevier B.V. All rights reserved.
ISSN: 13835718
DOI: 10.1016/j.mrgentox.2012.04.012

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