Divergent Roles of Salmonella Pathogenicity Island 2 and Metabolic Traits during Interaction of S. enterica Serovar Typhimurium with Host Cells

DC FieldValueLanguage
dc.contributor.authorHoelzer, Stefanie U.
dc.contributor.authorHensel, Michael
dc.date.accessioned2021-12-23T16:08:49Z-
dc.date.available2021-12-23T16:08:49Z-
dc.date.issued2012
dc.identifier.issn19326203
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/8472-
dc.description.abstractThe molecular mechanisms of virulence of the gastrointestinal pathogen Salmonella enterica are commonly studied using cell culture models of infection. In this work, we performed a direct comparison of the interaction of S. enterica serovar Typhimurium (S. Typhimurium) with the non-polarized epithelial cell line HeLa, the polarized cell lines CaCo2, T84 and MDCK, and macrophage-like RAW264.7 cells. The ability of S. Typhimurium wild-type and previously characterized auxotrophic mutant strains to enter host cells, survive and proliferate within mammalian cells and deploy the Salmonella Pathogenicity Island 2-encoded type III secretion system (SPI2-T3SS) was quantified. We found that the entry of S. Typhimurium into polarized cells was much more efficient than entry into non-polarized cells or phagocytic uptake. While SPI2-T3SS dependent intracellular proliferation was observed in HeLa and RAW cells, the intracellular replication in polarized cells was highly restricted and not affected by defective SPI2-T3SS. The contribution of aromatic amino acid metabolism and purine biosynthesis to intracellular proliferation was distinct in the various cell lines investigated. These observations indicate that the virulence phenotypes of S. Typhimurium are significantly affected by the cell culture model applied.
dc.description.sponsorshipDFG (Deutsche Forschungsgemeinschaft)German Research Foundation (DFG) [HE1964/14-1, 1316]; State of Bavaria; This work was supported by grant HE1964/14-1 as part of the DFG priority program (Deutsche Forschungsgemeinschaft) 1316 `Host-adapted metabolism of bacterial pathogens'. SUH was participant of the elite graduate school on `Lead Structures of Cell Function' funded by the State of Bavaria. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language.isoen
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.ispartofPLOS ONE
dc.subjectDENDRITIC CELLS
dc.subjectEFFECTOR PROTEINS
dc.subjectIDENTIFICATION
dc.subjectIII SECRETION SYSTEM
dc.subjectMACROPHAGES
dc.subjectMODEL
dc.subjectMultidisciplinary Sciences
dc.subjectPROLIFERATION
dc.subjectScience & Technology - Other Topics
dc.subjectSURVIVAL
dc.subjectTRANSLOCATION
dc.subjectVIRULENCE FACTORS
dc.titleDivergent Roles of Salmonella Pathogenicity Island 2 and Metabolic Traits during Interaction of S. enterica Serovar Typhimurium with Host Cells
dc.typejournal article
dc.identifier.doi10.1371/journal.pone.0033220
dc.identifier.isiISI:000302381500114
dc.description.volume7
dc.description.issue3
dc.publisher.place1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
dcterms.isPartOf.abbreviationPLoS One
dcterms.oaStatusGreen Published, gold, Green Submitted
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-6604-6253-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidHeMi480-
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