Regulation of nleA in Shiga Toxin-Producing Escherichia coli O84:H4 Strain 4795/97

DC FieldValueLanguage
dc.contributor.authorSchwidder, Maike
dc.contributor.authorHensel, Michael
dc.contributor.authorSchmidt, Herbert
dc.date.accessioned2021-12-23T16:08:50Z-
dc.date.available2021-12-23T16:08:50Z-
dc.date.issued2011
dc.identifier.issn00219193
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/8479-
dc.description.abstractMany Shiga toxin-producing Escherichia coli (STEC) strains express a type III secretion system (TTSS) encoded by the locus of enterocyte effacement (LEE). Using the TTSS, STEC is able to inject effector proteins directly into eukaryotic host cells, where they cause characteristic attaching and effacing (A/E) lesions. In addition to the LEE-encoded effectors, a number of non-LEE-encoded effectors, located on phage-associated elements, have been described. One of them, the non-LEE-encoded effector A (NleA), is widely distributed among pathogenic E. coli. In this study, we investigated the influence of environmental conditions on the expression of the phage-encoded effector nleA gene (designated nleA(4795)) present in STEC O84:H4 strain 4795/97. We demonstrated that a particular NaCl concentration and starvation stress increase the activity of the nleA(4795) promoter. Moreover, several regulators that control nleA(4795) expression were identified. The involvement of the LEE regulators Ler, GrlA, and GrlR show that nleA(4795) is integrated in the LEE regulation circuit. Furthermore, the binding of Ler to sequences upstream of nleA(4795) underlined these findings.
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG) [Schm 1360/3-1]; This work was supported by grant Schm 1360/3-1 of the Deutsche Forschungsgemeinschaft (DFG).
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofJOURNAL OF BACTERIOLOGY
dc.subjectCITROBACTER-RODENTIUM
dc.subjectCONTROLS EXPRESSION
dc.subjectENTEROCYTE EFFACEMENT GENES
dc.subjectIII EFFECTOR PROTEIN
dc.subjectLEE PATHOGENICITY ISLAND
dc.subjectLOCUS
dc.subjectMicrobiology
dc.subjectO157-H7
dc.subjectSECRETION
dc.subjectVIBRIO-HARVEYI
dc.subjectVIRULENCE FACTOR NLEA
dc.titleRegulation of nleA in Shiga Toxin-Producing Escherichia coli O84:H4 Strain 4795/97
dc.typejournal article
dc.identifier.doi10.1128/JB.00582-10
dc.identifier.isiISI:000286595600005
dc.description.volume193
dc.description.issue4
dc.description.startpage832
dc.description.endpage841
dc.contributor.orcid0000-0001-6046-4198
dc.identifier.eissn10985530
dc.publisher.place1752 N ST NW, WASHINGTON, DC 20036-2904 USA
dcterms.isPartOf.abbreviationJ. Bacteriol.
dcterms.oaStatusGreen Published, Bronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-6604-6253-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidHeMi480-
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