The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo

DC FieldValueLanguage
dc.contributor.authorDupres, Vincent
dc.contributor.authorAlsteens, David
dc.contributor.authorWilk, Sabrina
dc.contributor.authorHansen, Benjamin
dc.contributor.authorHeinisch, Juergen J.
dc.contributor.authorDufrene, Yves F.
dc.date.accessioned2021-12-23T16:08:52Z-
dc.date.available2021-12-23T16:08:52Z-
dc.date.issued2009
dc.identifier.issn15524450
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/8489-
dc.description.abstractHere we report on in vivo measurement of the mechanical behavior of a cell surface sensor using single-molecule atomic force microscopy. We focus on the yeast wall stress component sensor Wsc1, a plasma membrane protein that is thought to function as a rigid probe of the cell wall status. We first map the distribution of individual histidine-tagged sensors on living yeast cells by scanning the cell surface with atomic force microscopy tips carrying nitrilotriacetate groups. We then show that Wsc1 behaves like a linear nanospring that is capable of resisting high mechanical force and of responding to cell surface stress. Both a genomic pmt4 deletion and the insertion of a stretch of glycines in Wsc1 result in substantial alterations in protein spring properties, supporting the important role of glycosylation at the extracellular serine/threonine-rich region.
dc.description.sponsorshipBelgian National Foundation for Scientific Research (FNRS)Fonds de la Recherche Scientifique - FNRS; Universite catholique de Louvain (Fonds Speciaux de Recherche); Federal Office for Scientific, Technical and Cultural Affairs; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB431]; This work was supported by the Belgian National Foundation for Scientific Research (FNRS), the Universite catholique de Louvain (Fonds Speciaux de Recherche), the Region wallonne, the Federal Office for Scientific, Technical and Cultural Affairs (Interuniversity Poles of Attraction Programme), and the Research Department of the Communaute francaise de Belgique (Concerted Research Action). Work at the University of Osnabruck was funded by the Deutsche Forschungsgemeinschaft within the framework of the SFB431.
dc.language.isoen
dc.publisherNATURE PUBLISHING GROUP
dc.relation.ispartofNATURE CHEMICAL BIOLOGY
dc.subjectATOMIC-FORCE MICROSCOPY
dc.subjectBiochemistry & Molecular Biology
dc.subjectDOMAINS
dc.subjectINTEGRITY
dc.subjectMID2
dc.subjectPATHWAYS
dc.subjectPROTEIN
dc.subjectRECEPTOR
dc.subjectSACCHAROMYCES-CEREVISIAE
dc.subjectSINGLE-MOLECULAR RECOGNITION
dc.subjectSPECTROSCOPY
dc.titleThe yeast Wsc1 cell surface sensor behaves like a nanospring in vivo
dc.typejournal article
dc.identifier.doi10.1038/nchembio.220
dc.identifier.isiISI:000270915000016
dc.description.volume5
dc.description.issue11
dc.description.startpage857
dc.description.endpage862
dc.contributor.orcid0000-0001-9229-113X
dc.contributor.orcid0000-0001-9229-113X
dc.contributor.orcid0000-0002-7289-4248
dc.contributor.researcheridAAU-5475-2020
dc.contributor.researcheridF-2669-2014
dc.contributor.researcheridG-3801-2017
dc.identifier.eissn15524469
dc.publisher.place75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA
dcterms.isPartOf.abbreviationNat. Chem. Biol.
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