Functional characterization of the maltose ATP-binding-cassette transporter of Salmonella typhimurium by means of monoclonal antibodies directed against the Ma1K subunit

Autor(en): Stein, A
Seifert, M
Volkmer-Engert, R
Siepelmeyer, J
Jahreis, K
Schneider, E 
Stichwörter: ABC TRANSPORTER; Biochemistry & Molecular Biology; COLI MAL REGULON; CONFORMATIONAL-CHANGES; CRYSTAL-STRUCTURE; enzyme IIA(G1c); ESCHERICHIA-COLI; INDUCER EXCLUSION; Ma1T; MalFGK2; MEMBRANE SUPPORTS; monoclonal antibodies; PHOSPHOTRANSFERASE SYSTEM; PROTEIN MALK; SPOT-SYNTHESIS
Erscheinungsdatum: 2002
Herausgeber: BLACKWELL PUBLISHING LTD
Journal: EUROPEAN JOURNAL OF BIOCHEMISTRY
Volumen: 269
Ausgabe: 16
Startseite: 4074
Seitenende: 4085
Zusammenfassung: 
The maltose ATP-binding cassette transporter of Salmonella typhimurium is composed of a membrane-associated complex (MalFGK(2)) and a periplasmic receptor (MalE). In addition to its role in transport, the complex acts as a repressor of maltose-regulated gene expression and is subject to inhibition in the process of inducer exclusion. These activities are thought to be mediated by interactions of the ATPase subunit, MalK, with the transcriptional activator, MaIT, and nonphosphorylated enzyme IIA of the glucose phosphotransferase system, respectively. To gain further insight in protein regions that are critical for these functions, we have generated nine MaIK-specific monoclonal antibodies. These bind to four nonoverlapping linear epitopes: 60-LFig-63 (5B5), 113-RVNQVAEVLQL-123 (represented by 4H12), 309-GHETQI-314 (2179) and 352-LFREDG SACR-361 (represented by 4133). All mAbs recognize their epitopes in soluble MalK and in the MalFGK(2) complex with K-d values ranging from 10(-6) to 10(-8) m. ATP reduced the affinity of the mAbs for soluble MalK, indicating a conformational change that renders the epitopes less accessible. 4H12 and 5B5 inhibit the ATPase activity of MalK and the MalE/maltose-stimulated ATPase activity of proteoliposomes, while their Fab fragments displayed no significant effect. The results suggest a similar solvent-exposed position of helix 3 in the MalK dimer and in the intact complex and might argue against a direct role in the catalytic process. 4B3 and 2F9 exhibit reduced binding to the MalFGK(2) Complex in the presence of MalT and enzyme IIA(Glc), respectively, thereby providing the first direct evidence for the C-terminal domain of MalK being the site of interaction with the regulatory proteins.
ISSN: 00142956
DOI: 10.1046/j.1432-1033.2002.03099.x

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