Transient genetic transformation of Mougeotia scalaris (Zygnematophyceae) mediated by the endogenous alpha-tubulin1 promoter
| Autor(en): | Regensdorff, Merle Deckena, Marek Stein, Maike Borchers, Agnes Scherer, Gwydion Lammers, Maike Haensch, Robert Zachgo, Sabine Buschmann, Henrik |
Stichwörter: | biolistic particle delivery; cell division; CELL-WALL; CHLOROPHYTA; EVOLUTION; EXPRESSION; genetic transformation; green fluorescent protein; GREEN-ALGA MOUGEOTIA; Marine & Freshwater Biology; Mougeotia scalaris; ORIGIN; PHYLOGENIES; Plant Sciences; PLANTS; REGENERATING PROTOPLASTS; streptophyte algae; TERRESTRIAL; tubulin | Erscheinungsdatum: | 2018 | Herausgeber: | WILEY | Journal: | JOURNAL OF PHYCOLOGY | Volumen: | 54 | Ausgabe: | 6 | Startseite: | 840 | Seitenende: | 849 | Zusammenfassung: | Mougeotia scalaris is a filamentous streptophyte alga renowned for light-inducible plastid rotation and microtubule-dependent polarity establishment. As a first step toward transgenic approaches we determined the 5,825 base pair genomic sequence encoding the alpha-tubulin1 gene (MsTUA1) of M. scalaris (strain SAG 164.80). The subcloned MsTUA1 promoter facilitated strong transgene expression in M. scalaris and tobacco leaf cells, as shown by particle bombardment and the subsequent visualization of expressed fluorescent protein markers. Our results provide a route for the genetic transformation of the filamentous streptophyte alga M. scalaris based on the endogenous TUA1 promoter. |
ISSN: | 00223646 | DOI: | 10.1111/jpy.12781 |
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