Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity

Autor(en): Ulmke, C
Kreth, J
Lengeler, JW
Welte, W
Schmid, K
Stichwörter: CHANNEL; CLONING; ESCHERICHIA-COLI; GENE; MALTOPORIN; METABOLISM; Microbiology; OUTER-MEMBRANE; STRUCTURAL BASIS; SUGAR TRANSLOCATION; TRANSPORT
Erscheinungsdatum: 1999
Herausgeber: AMER SOC MICROBIOLOGY
Journal: JOURNAL OF BACTERIOLOGY
Volumen: 181
Ausgabe: 6
Startseite: 1920
Seitenende: 1923
Zusammenfassung: 
The difference in substrate selectivity of the maltodextrin (LamB) and sucrose (ScrY) porins is attributed mainly to differences in loop L3, which is supposed to constrict the lumen of the pores. We show that even a single mutation (D201Y) in loop L3 leads to a narrowing of the substrate range of ScrY to that resembling LamB. In addition, we removed the putative N-terminal coiled-coil structure of ScrY and studied the effect of this deletion on sucrose transport.
ISSN: 00219193
DOI: 10.1128/JB.181.6.1920-1923.1999

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