DC Field | Value | Language |
dc.contributor.author | Gao, Jieqiong | |
dc.contributor.author | Kurre, Rainer | |
dc.contributor.author | Rose, Jaqueline | |
dc.contributor.author | Walter, Stefan | |
dc.contributor.author | Froehlich, Florian | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | Reggiori, Fulvio | |
dc.contributor.author | Ungermann, Christian | |
dc.date.accessioned | 2021-12-23T16:09:06Z | - |
dc.date.available | 2021-12-23T16:09:06Z | - |
dc.identifier.issn | 1469221X | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/8620 | - |
dc.description.abstract | The mechanism and regulation of fusion between autophagosomes and lysosomes/vacuoles are still only partially understood in both yeast and mammals. In yeast, this fusion step requiresSNAREproteins, the homotypic vacuole fusion and protein sorting (HOPS) tethering complex, theRAB7GTPase Ypt7, and its guanine nucleotide exchange factor (GEF) Mon1-Ccz1. We and others recently identified Ykt6 as the autophagosomalSNAREprotein. However, it has not been resolved when and how lipid-anchored Ykt6 is recruited onto autophagosomes. Here, we show that Ykt6 is recruited at an early stage of the formation of these carriers through a mechanism that depends on endoplasmic reticulum (ER)-resident Dsl1 complex andCOPII-coated vesicles. Importantly, Ykt6 activity on autophagosomes is regulated by the Atg1 kinase complex, which inhibits Ykt6 through direct phosphorylation. Thus, our findings indicate that the Ykt6 pool on autophagosomal membranes is kept inactive by Atg1 phosphorylation, and once an autophagosome is ready to fuse with vacuole, Ykt6 dephosphorylation allows its engagement in the fusion event. | |
dc.description.sponsorship | ALW Open Program [ALWOP.310]; ZonMW TOP [91217002]; Open Competition ENW-KLEIN [OCENW.KLEIN.118]; Marie Skodowska-Curie [713660, 765912]; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [UN111/7-3, 13-1, Sonderforschungsbereich 944]; DFGGerman Research Foundation (DFG)European Commission; Projekt DEAL; We thank Angela Perz and Kathrin Auffarth for expert technical assistance. F. Reggiori was supported by ALW Open Program (ALWOP.310), ZonMW TOP (91217002), an Open Competition ENW-KLEIN (OCENW.KLEIN.118), Marie Skodowska-Curie Cofund (713660), and Marie Skodowska-Curie ITN (765912) grants. C. Ungermann was supported by a grant of the Deutsche Forschungsgemeinschaft (UN111/7-3 and 13-1) and the Sonderforschungsbereich 944 (Project P11). R. Kurre and J. Piehler were supported by the Sonderforschungsbereich 944 (Projekt Z01) and the iBiOs funds of the DFG. Open access funding enabled and organized by Projekt DEAL. | |
dc.language.iso | en | |
dc.publisher | WILEY | |
dc.relation.ispartof | EMBO REPORTS | |
dc.subject | autophagy | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Cell Biology | |
dc.subject | COPIIvesicles | |
dc.subject | Dsl1 complex | |
dc.subject | EARLY STEPS | |
dc.subject | EARLY-STAGE | |
dc.subject | ENDOPLASMIC RETICULUM RETRIEVAL | |
dc.subject | GOLGI | |
dc.subject | R-SNARE | |
dc.subject | SACCHAROMYCES-CEREVISIAE | |
dc.subject | SNARE | |
dc.subject | SNARE PROTEINS | |
dc.subject | TETHERING COMPLEX | |
dc.subject | VESICLE FORMATION | |
dc.subject | YEAST | |
dc.subject | Ykt6 | |
dc.title | Function of theSNAREYkt6 on autophagosomes requires the Dsl1 complex and the Atg1 kinase complex | |
dc.type | journal article | |
dc.identifier.doi | 10.15252/embr.202050733 | |
dc.identifier.isi | ISI:000575508300001 | |
dc.contributor.orcid | 0000-0003-2652-2686 | |
dc.contributor.orcid | 0000-0001-8307-2189 | |
dc.identifier.eissn | 14693178 | |
dc.publisher.place | 111 RIVER ST, HOBOKEN 07030-5774, NJ USA | |
dcterms.isPartOf.abbreviation | EMBO Rep. | |
dcterms.oaStatus | Green Published, hybrid | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-6872-6567 | - |
crisitem.author.orcid | 0000-0001-8307-2189 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | KuRa617 | - |
crisitem.author.netid | FrFl166 | - |
crisitem.author.netid | PiJa938 | - |
crisitem.author.netid | UnCh999 | - |