Evidence for two types of phosphate translocators in sweet-pepper (Capsicum annuum L) fruit chromoplasts

DC FieldValueLanguage
dc.contributor.authorQuick, WP
dc.contributor.authorNeuhaus, HE
dc.date.accessioned2021-12-23T16:09:14Z-
dc.date.available2021-12-23T16:09:14Z-
dc.date.issued1996
dc.identifier.issn02646021
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/8682-
dc.description.abstractWe have investigated whether there is evidence for the presence of different types of phosphate translocators in envelopes purified from pepper-fruit chromoplasts. A method was developed that allowed the purification of envelope membranes from isolated pepper-fruit chromoplasts. Proteoliposomes containing envelope-membrane proteins are able to import inorganic phosphate (P-i) or glucose 6-phosphate (Glc6P). In both cases, the rate of import is strongly dependent upon preloading of proteoliposomes with either P-i, dihydroxyacetone phosphate (DHAP) or Glc6P. This demonstrates the presence of a phosphate translocator activity catalysing a counter exchange of phosphorylated intermediates. Interestingly, a high external concentration of Glc6P does not strongly inhibit P-i uptake into proteoliposomes preloaded with DHAP, whereas external Glc6P strongly inhibits P-i uptake into proteoliposomes preloaded with Glc6P. This observation strongly indicates that two types of phosphate translocator are present in chromoplast envelopes from red-pepper fruits. These data are discussed with respect to the possible physiological function of two types of phosphate translocator in one type of plastid.
dc.language.isoen
dc.publisherPORTLAND PRESS
dc.relation.ispartofBIOCHEMICAL JOURNAL
dc.subjectAMYLOPLASTS
dc.subjectBiochemistry & Molecular Biology
dc.subjectCHLOROPLAST ENVELOPE
dc.subjectGLUCOSE-6-PHOSPHATE
dc.subjectINNER MEMBRANE
dc.subjectINORGANIC-PHOSPHATE
dc.subjectPISUM-SATIVUM-L
dc.subjectPLASTIDS
dc.subjectSPINACH-CHLOROPLASTS
dc.subjectSTARCH SYNTHESIS
dc.subjectTRANSPORT
dc.titleEvidence for two types of phosphate translocators in sweet-pepper (Capsicum annuum L) fruit chromoplasts
dc.typejournal article
dc.identifier.isiISI:A1996VU84500002
dc.description.volume320
dc.description.issue1
dc.description.startpage7
dc.description.endpage10
dc.publisher.place59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ
dcterms.isPartOf.abbreviationBiochem. J.
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