Biochemical identification of a lipoprotein with maltose-binding activity in the thermoacidophilic Gram-positive bacterium Alicyclobacillus acidocaldarius
Autor(en): | Herrmann, A Schlosser, A Schmid, R Schneider, E |
Stichwörter: | Alicyclobacillus acidocaldarius; AMYLASE; BACILLUS-ACIDOCALDARIUS; binding protein-dependent transport system; ESCHERICHIA-COLI; Gram(+) bacteria; maltose; maltose uptake; Microbiology; PROTEIN; thermoacidophile; TRANSPORT | Erscheinungsdatum: | 1996 | Herausgeber: | EDITIONS SCIENTIFIQUES ELSEVIER | Journal: | RESEARCH IN MICROBIOLOGY | Volumen: | 147 | Ausgabe: | 9 | Startseite: | 733 | Seitenende: | 737 | Zusammenfassung: | Growth of the thermoacidophilic Gram-positive bacterium Alicyclobacillus acidocaldarius strain ATCC 27009 on maltose resulted in the increased production of a protein with apparent molecular mass of 40 kDa. By metabolic labelling with C-14-palmitic acid, the 40-kDa protein was identified as a lipoprotein. The protein exhibited maltose-binding activity at pH 3.5, as demonstrated by chromatography on cross-linked amylose. Partial amino acid sequence analysis revealed that the 40-kDa protein coresponds to the product of an open reading frame downstream from the amylase gene (amy) that displays similarity to enterobacterial maltose-binding proteins. |
ISSN: | 09232508 | DOI: | 10.1016/S0923-2508(97)85120-0 |
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