The 36K protein of zebrafish CNS myelin is a short-chain dehydrogenase

DC FieldValueLanguage
dc.contributor.authorMorris, JK
dc.contributor.authorWillard, BB
dc.contributor.authorYin, XH
dc.contributor.authorJeserich, G
dc.contributor.authorKinter, M
dc.contributor.authorTrapp, BD
dc.date.accessioned2021-12-23T16:09:20Z-
dc.date.available2021-12-23T16:09:20Z-
dc.date.issued2004
dc.identifier.issn08941491
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/8737-
dc.description.abstractPrevious studies identified homologues to mammalian myelin genes expressed in the teleost central nervous system (CNS), including myelin basic protein (MBP), protein zero (PO), and a member of the proteolipid protein family, DM20. In addition, an uncharacterized 36-kDa (36K) protein is a major component of teleost myelin, but is not a major component of myelin in other species. In the present study, we sought to better understand myelin proteins and myelination in one teleost, zebrafish, by molecular characterization of the zebrafish 36K protein. Purified zebrafish CNS myelin was isolated and the amino acid sequences of peptides present in the 36-kDa band were determined by mass spectrometry. These sequences matched a previously uncharacterized EST in The Institute for Genome Research (TIGR) zebrafish database that is related to the short-chain dehydrogenase/reductase (SDR) protein family. In vitro expression of the zebrafish 36K cDNA in Neuro 2a cells resulted in a protein product that was recognized by a 36K polyclonal antibody. The zebrafish 36K mRNA and protein expression patterns were determined and correlated to other known myelin gene expression profiles. In addition, we determined by in situ hybridization that a human 36K homologue (FLJ13639) is expressed in oligodendrocytes and neurons in the adult human cortex. This study identified a major myelin protein in zebrafish, 36K, as a member of the SDR superfamily; an expression pattern similar to other myelin genes was demonstrated. (C) 2003 Wiley-Liss, Inc.
dc.description.sponsorshipNINDS NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Neurological Disorders & Stroke (NINDS) [R01 NS 38186] Funding Source: Medline; NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKEUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Neurological Disorders & Stroke (NINDS) [R01NS038186] Funding Source: NIH RePORTER
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofGLIA
dc.subject2',3'-CYCLIC NUCLEOTIDE 3'-PHOSPHODIESTERASE
dc.subjectBASIC-PROTEIN
dc.subjectCENTRAL-NERVOUS-SYSTEM
dc.subjectdevelopment
dc.subjectFISH MYELIN
dc.subjectIMMUNOHISTOCHEMICAL LOCALIZATION
dc.subjectMESSENGER-RNA
dc.subjectMOLECULAR-CLONING
dc.subjectmyelination
dc.subjectNA,K-ATPASE ALPHA-SUBUNIT
dc.subjectNeurosciences
dc.subjectNeurosciences & Neurology
dc.subjectoligodendrocyte
dc.subjectPROTEOLIPID PROTEIN
dc.subjectshort-chain dehydrogenase
dc.subjectTISSUE EXPRESSION
dc.titleThe 36K protein of zebrafish CNS myelin is a short-chain dehydrogenase
dc.typejournal article
dc.identifier.doi10.1002/glia.10338
dc.identifier.isiISI:000189284400007
dc.description.volume45
dc.description.issue4
dc.description.startpage378
dc.description.endpage391
dc.contributor.orcid0000-0002-3515-0821
dc.contributor.orcid0000-0001-7409-8918
dc.identifier.eissn10981136
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationGlia
Show simple item record

Page view(s)

2
Last Week
0
Last month
0
checked on Apr 15, 2024

Google ScholarTM

Check

Altmetric