Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside

DC FieldValueLanguage
dc.contributor.authorNauwelaerts, K
dc.contributor.authorVastmans, K
dc.contributor.authorFroeyen, M
dc.contributor.authorKempeneers, V
dc.contributor.authorRozenski, J
dc.contributor.authorRosemeyer, H
dc.contributor.authorVan Aerschot, A
dc.contributor.authorBusson, R
dc.contributor.authorLacey, JC
dc.contributor.authorEfimtseva, E
dc.contributor.authorMikhailov, S
dc.contributor.authorLescrinier, E
dc.contributor.authorHerdewijn, P
dc.date.accessioned2021-12-23T16:09:20Z-
dc.date.available2021-12-23T16:09:20Z-
dc.date.issued2003
dc.identifier.issn03051048
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/8740-
dc.description.abstractA ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.
dc.language.isoen
dc.publisherOXFORD UNIV PRESS
dc.relation.ispartofNUCLEIC ACIDS RESEARCH
dc.subjectBiochemistry & Molecular Biology
dc.subjectCONTACTS
dc.subjectCOUPLING-CONSTANTS
dc.subjectHAIRPIN FORMATION
dc.subjectNMR-SPECTROSCOPY
dc.subjectNUCLEIC-ACIDS
dc.subjectNUCLEOTIDES
dc.subjectOLIGONUCLEOTIDES
dc.subjectPOLYMERASE-I
dc.subjectPROTEINS
dc.subjectPROTON NMR
dc.titleCleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside
dc.typejournal article
dc.identifier.doi10.1093/nar/gkg911
dc.identifier.isiISI:000186802500014
dc.description.volume31
dc.description.issue23
dc.description.startpage6758
dc.description.endpage6769
dc.contributor.orcid0000-0002-8675-6540
dc.contributor.orcid0000-0002-1363-9706
dc.contributor.orcid0000-0001-7066-4329
dc.contributor.orcid0000-0003-3589-8503
dc.contributor.orcid0000-0001-9624-5536
dc.contributor.orcid0000-0003-3528-8588
dc.contributor.researcheridI-1665-2014
dc.contributor.researcheridF-3414-2013
dc.contributor.researcheridG-9339-2016
dc.publisher.placeGREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
dcterms.isPartOf.abbreviationNucleic Acids Res.
dcterms.oaStatusGreen Published, Green Submitted, Bronze
crisitem.author.deptInstitut für Chemie neuer Materialien-
crisitem.author.deptidinstitute11-
crisitem.author.parentorgFB 05 - Biologie/Chemie-
crisitem.author.grandparentorgUniversität Osnabrück-
crisitem.author.netidRoHe783-
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