Evidence that an evolutionary transition from dehiscent to indehiscent fruits in Lepidium (Brassicaceae) was caused by a change in the control of valve margin identity genes
DC Element | Wert | Sprache |
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dc.contributor.author | Muehlhausen, Andreas | |
dc.contributor.author | Lenser, Teresa | |
dc.contributor.author | Mummenhoff, Klaus | |
dc.contributor.author | Theissen, Guenter | |
dc.date.accessioned | 2021-12-23T16:09:28Z | - |
dc.date.available | 2021-12-23T16:09:28Z | - |
dc.date.issued | 2013 | |
dc.identifier.issn | 09607412 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/8814 | - |
dc.description.abstract | In the Brassicaceae, indehiscent fruits evolved from dehiscent fruits several times independently. Here we use closely related wild species of the genus Lepidium as a model system to analyse the underlying developmental genetic mechanisms in a candidate gene approach. ALCATRAZ (ALC), INDEHISCENT (IND), SHATTERPROOF1 (SHP1) and SHATTERPROOF2 (SHP2) are known fruit developmental genes of Arabidopsis thaliana that are expressed in the fruit valve margin governing dehiscence zone formation. Comparative expression analysis by quantitative RT-PCR, Northern blot and in situ hybridization show that their orthologues from Lepidium campestre (dehiscent fruits) are similarly expressed at valve margins. In sharp contrast, expression of the respective orthologues is abolished in the corresponding tissue of indehiscent Lepidium appelianum fruits, indicating that changes in the genetic pathway identified in A.thaliana caused the transition from dehiscent to indehiscent fruits in the investigated species. As parallel mutations in different genes are quite unlikely, we conclude that the changes in gene expression patterns are probably caused by changes in upstream regulators of ALC, IND and SHP1/2, possible candidates from A.thaliana being FRUITFULL (FUL), REPLUMLESS (RPL) and APETALA2 (AP2). However, neither expression analyses nor functional tests in transgenic plants provided any evidence that the FUL or RPL orthologues of Lepidium were involved in evolution of fruit indehiscence in Lepidium. In contrast, stronger expression of AP2 in indehiscent compared to dehiscent fruits identifies AP2 as a candidate gene that deserves further investigation. | |
dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [MU 1137/8-1, TH 417/6-1]; Universitatsgesellschaft Osnabruck; We thank Ulrike Coja for technical assistance, the staff of the Botanical Garden Osnabruck for plant cultivation, and Lucille Schmieding for correcting style and grammar. We appreciated access to the in situ facilities of the Zachgo laboratory (Department of Biology/Chemistry, University of Osnabruck, Germany). Many thanks also to Pia Nutt and Dajana Lobbes for help with in situ hybridization analyses, to Mariana Mondragon-Palomino for introduction to quantitative RT-PCR, to Lydia Gramzow for support with bioinformatics questions, to Domenica Schnabelrauch (Departmant of Entomology, MPI for Chemical Ecology, Jena, Germany) for her sequencing efforts, and to Nicolas Arnaud and Robert Sablowski (Department of Cell & Developmental Biology, John Innes Centre, Norwich, UK) for valuable information. We are also very grateful to two anonymous reviewers for their helpful comments on a previous version of the manuscript. This work was supported by grants from the Deutsche Forschungsgemeinschaft to K.M. (MU 1137/8-1) and G.T. (TH 417/6-1), and by a grant from the Universitatsgesellschaft Osnabruck to K.M. | |
dc.language.iso | en | |
dc.publisher | WILEY | |
dc.relation.ispartof | PLANT JOURNAL | |
dc.subject | ARABIDOPSIS | |
dc.subject | Brassicaceae | |
dc.subject | CELL FATE | |
dc.subject | character evolution | |
dc.subject | evolutionary developmental biology | |
dc.subject | FLOWER DEVELOPMENT | |
dc.subject | fruit dehiscence | |
dc.subject | HOMEODOMAIN PROTEIN | |
dc.subject | L epidium appelianum | |
dc.subject | L epidium campestre | |
dc.subject | MADS-BOX GENES | |
dc.subject | PHYLOGENY | |
dc.subject | Plant Sciences | |
dc.subject | POD SHATTER | |
dc.subject | SEED DISPERSAL | |
dc.subject | TRANSCRIPTION | |
dc.subject | valve margin | |
dc.subject | ZINC-FINGER | |
dc.title | Evidence that an evolutionary transition from dehiscent to indehiscent fruits in Lepidium (Brassicaceae) was caused by a change in the control of valve margin identity genes | |
dc.type | journal article | |
dc.identifier.doi | 10.1111/tpj.12079 | |
dc.identifier.isi | ISI:000315359600010 | |
dc.description.volume | 73 | |
dc.description.issue | 5 | |
dc.description.startpage | 824 | |
dc.description.endpage | 835 | |
dc.contributor.orcid | 0000-0002-8449-1593 | |
dc.identifier.eissn | 1365313X | |
dc.publisher.place | 111 RIVER ST, HOBOKEN 07030-5774, NJ USA | |
dcterms.isPartOf.abbreviation | Plant J. | |
dcterms.oaStatus | Bronze | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | Universität Osnabrück | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-8449-1593 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | MuKl919 | - |
crisitem.author.netid | ThGu001 | - |
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