The phosphoenolpyruvate-dependent glucose-phosphotransferase system from Escherichia coli K-12 as the center of a network regulating carbohydrate flux in the cell
Autor(en): | Gabor, Elisabeth Goehler, Anna-Katharina Kosfeld, Anne Staab, Ariane Kremling, Andreas Jahreis, Knut |
Stichwörter: | Bacterial phosphotransferase system; Carbohydrate transport; CATABOLITE REPRESSION; Cell Biology; CYSTEINE CROSS-LINKING; Gene regulation; GLOBAL REPRESSOR MLC; Glucose; Integral membrane protein; MANNITOL PERMEASE; MEMBRANE SEQUESTRATION; Metabolic balance; Mlc; Modeling; MtfA; PIS; POSTTRANSCRIPTIONAL REGULATION; ptsG; SgrT; SIGNAL-TRANSDUCTION; SUBSTRATE-SPECIFICITY; TRANSPORTER MESSENGER-RNA; UNCOUPLE TRANSPORT | Erscheinungsdatum: | 2011 | Herausgeber: | ELSEVIER GMBH, URBAN & FISCHER VERLAG | Journal: | EUROPEAN JOURNAL OF CELL BIOLOGY | Volumen: | 90 | Ausgabe: | 9 | Startseite: | 711 | Seitenende: | 720 | Zusammenfassung: | The phosphoenolpyruvate-(PEP)-dependent-carbohydrate:phosphotransferase systems (PTSs) of enteric bacteria constitute a complex transport and sensory system. Such a PTS usually consists of two cytoplasmic energy-coupling proteins, Enzyme I (El) and HPr, and one of more than 20 different carbohydrate-specific membrane proteins named Enzyme II (Ell), which catalyze the uptake and concomitant phosphorylation of numerous carbohydrates. The most prominent representative is the glucose-PTS, which uses a PTS-typical phosphcrylation cascade to transport and phosphorylate glucose. All components of the glucose-PTS interact with a large number of non-PTS proteins to regulate the carbohydrate flux in the bacterial cell. Several aspects of the glucose-PTS have been intensively investigated in various research projects of many groups. In this article we will review our recent findings on a Glc-PTS-dependent metalloprotease, on the interaction of EIICBGlc with the regulatory peptide SgrT, on the structure of the membrane spanning C-domain of the glucose transporter and on the modeling approaches of ptsG regulation, respectively, and discuss them in context of general PTS research. (C) 2011 Elsevier GmbH. All rights reserved. |
ISSN: | 01719335 | DOI: | 10.1016/j.ejcb.2011.04.002 |
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