Cryo-EM of ABC transporters: an ice-cold solution to everything?

Autor(en): Januliene, Dovile 
Moeller, Arne 
Stichwörter: ABC transporters; BILAYER NANODISCS; Biochemistry & Molecular Biology; Biophysics; Cell Biology; CFTR; conformational spectrum; cryo‐ CRYOELECTRON MICROSCOPY; EM; GRID PREPARATION; membrane proteins; MEMBRANE-PROTEINS; MOLECULAR-STRUCTURE; RECONSTRUCTION; SOLUBILIZATION; STRUCTURAL BASIS; SUBSTRATE
Erscheinungsdatum: 2020
Herausgeber: WILEY
Volumen: 594
Ausgabe: 23, SI
Startseite: 3776
Seitenende: 3789
High-resolution cryo-EM has revolutionized how we look at ABC transporters and membrane proteins in general. An ever-increasing number of software tools and faster processing now allow dissecting the molecular details of nanomachines at atomic precision. Considering the further benefits of significantly reduced sample demands and increased speed, cryo-EM will dominate the structure determination of membrane proteins in the near future without compromising on data quality or detail. Moreover, improved and new algorithms make it now possible to resolve the conformational spectrum of macromolecular machines under turnover conditions and to analyze heterogeneous samples at high resolution. The future of cryo-EM is, therefore, bright, and the growing number of imaging facilities and groups active in this field will amplify this trend even further. Nevertheless, expectations have to be managed, as cryo-EM alone cannot provide an ultimate answer to all scientific questions. In this review, we discuss the capabilities and limitations of cryo-EM together with possible solutions for studies of ABC transporters.
ISSN: 00145793
DOI: 10.1002/1873-3468.13989

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