A Bnr-like formin links actin to the spindle pole body during sporulation in the filamentous fungus Ashbya gossypii

Autor(en): Kemper, Michael
Mohlzahn, Lars
Lickfeld, Manuela
Lang, Claudia
Waehlisch, Sabrina
Schmitz, Hans-Peter 
Stichwörter: Biochemistry & Molecular Biology; CORE PROTEINS; FORESPORE MEMBRANE; GENE-EXPRESSION; MEIOTIC SPINDLE; Microbiology; NUCLEATION; PROSPORE MEMBRANE; SACCHAROMYCES-CEREVISIAE; SCHIZOSACCHAROMYCES-POMBE; TRANSFORMATION; YEAST
Erscheinungsdatum: 2011
Herausgeber: WILEY
Journal: MOLECULAR MICROBIOLOGY
Volumen: 80
Ausgabe: 5
Startseite: 1276
Seitenende: 1295
Zusammenfassung: 
P>Formin proteins are nucleators of actin filaments and regulators of the microtubule cytoskeleton. As such, they play important roles in the development of yeast and other fungi. We show here that AgBnr2, a homologue of the ScBnr1 formin from the filamentous fungus Ashbya gossypii, localizes to the spindle pole body (SPB), the fungal analogue of the centrosome of metazoans. This protein plays an important role in the development of the typical needle-shaped spores of A. gossypii, as suggested by several findings. First, downregulation of AgBNR2 causes defects in sporangium formation and a decrease in the total spore number. Second, a fusion of AgBNR2 to GFP that is driven by the native AgBNR2 promoter is only visible in sporangia. Third, AgBnr2 interacts with a AgSpo21, a sporulation-specific component of the SPB. Furthermore, we provide evidence that AgBnr2 might nucleate actin cables, which are connected to SPBs during sporulation. Our findings add to our understanding of fungal sporulation, particularly the formation of spores with a complex, elongated morphology, and provide novel insights into formin function.
ISSN: 0950382X
DOI: 10.1111/j.1365-2958.2011.07644.x

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