High-field EPR and site-directed spin labeling reveal a periodical polarity profile: The sequence 88 to 94 of the phototransducer NpHtrII in complex with sensory rhodopsin, NpSRII

DC FieldValueLanguage
dc.contributor.authorBrutlach, H.
dc.contributor.authorBordignon, E.
dc.contributor.authorUrban, L.
dc.contributor.authorKlare, J. P.
dc.contributor.authorReyher, H. -J.
dc.contributor.authorEngelhard, M.
dc.contributor.authorSteinhoff, H. -J.
dc.date.accessioned2021-12-23T16:09:39Z-
dc.date.available2021-12-23T16:09:39Z-
dc.date.issued2006
dc.identifier.issn09379347
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/8919-
dc.description.abstractTaking advantage of the improved spectral resolution of high-field electron paramagnetic resonance (EPR) at 95 GHz/3.4 T as compared to conventional X-band EPR (9.5 GHz/0.34 T), detailed information on the polarity profile in a protein-protein interface is obtained. Nitroxide spin label side chains are introduced at positions 88 to 94 in the AS-1 sequence of the membrane adjacent HAMP domain of the transducer protein, NpHtrII, which is reconstituted in complex with sensory rhodopsin, NpSRII from Natronobacterium pharaonis. Position-dependent variations of the values of the nitroxide magnetic tenser components g(xx) and A(zz) suggest that the spin label side chains at positions 88 to 93 of AS-1 are located between a hydrophobic and a hydrophilic microenvironment. The observed periodicity of the polarity properties of the respective spin label microenvironment agrees with an a-helical secondary structure of this part of AS-1 and validates a recently published molecular model which locates residues 88 and 91 in the interface between helices F and G of NpSRII and AS-1 of NpHtrII close to the cytoplasmic lipid-water interface.
dc.language.isoen
dc.publisherSPRINGER WIEN
dc.relation.ispartofAPPLIED MAGNETIC RESONANCE
dc.subject95-GHZ
dc.subjectCHAINS
dc.subjectCONFORMATIONAL-CHANGES
dc.subjectPhysics
dc.subjectPhysics, Atomic, Molecular & Chemical
dc.subjectRESONANCE
dc.subjectSPECTROMETER
dc.subjectSpectroscopy
dc.titleHigh-field EPR and site-directed spin labeling reveal a periodical polarity profile: The sequence 88 to 94 of the phototransducer NpHtrII in complex with sensory rhodopsin, NpSRII
dc.typejournal article
dc.identifier.doi10.1007/BF03166206
dc.identifier.isiISI:000243548800008
dc.description.volume30
dc.description.issue3-4
dc.description.startpage359
dc.description.endpage372
dc.contributor.orcid0000-0002-5888-0157
dc.contributor.orcid0000-0002-5543-0812
dc.contributor.orcid0000-0002-5761-5968
dc.contributor.researcheridH-3791-2014
dc.contributor.researcheridF-1317-2010
dc.contributor.researcheridC-1428-2009
dc.identifier.eissn16137507
dc.publisher.placeSACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA
dcterms.isPartOf.abbreviationAppl. Magn. Reson.
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