Fluorescent DNA: the development of 7-deazapurine nucleoside triphosphates applicable for sequencing at the single molecule level
DC Element | Wert | Sprache |
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dc.contributor.author | Seela, F | |
dc.contributor.author | Feiling, E | |
dc.contributor.author | Gross, J | |
dc.contributor.author | Hillenkamp, F | |
dc.contributor.author | Ramzaeva, N | |
dc.contributor.author | Rosemeyer, H | |
dc.contributor.author | Zulauf, M | |
dc.date.accessioned | 2021-12-23T16:09:40Z | - |
dc.date.available | 2021-12-23T16:09:40Z | - |
dc.date.issued | 2001 | |
dc.identifier.issn | 01681656 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/8926 | - |
dc.description.abstract | 7-Deaza-2'-deoxyadenosine and -guanosine phosphoramidite building blocks as well as corresponding 5'-triphosphate derivatives are described carrying in position 7 substituents such as iodo, hexyn-1-yl or 5-aminopentyn-1-yl residues. The phosphoramidites were used to synthesize a series of modified oligodeoxynucleotides. A systematic study of the thermal stabilities of these oligonucleotide duplexes demonstrated that the 7-substituents are well accommodated in the major groove of B-DNA. The 7-(aminoalkyn-1-yl)-7-deazapurine 2'-deoxynucleoside triphosphates were labeled with bulky fluorophores such as Rhodamine Green (R) or tetramethylrhodamine. (C) 2001 Elsevier Science B.V. All rights reserved. | |
dc.language.iso | en | |
dc.publisher | ELSEVIER SCIENCE BV | |
dc.relation.ispartof | JOURNAL OF BIOTECHNOLOGY | |
dc.subject | 2'-DEOXYRIBOFURANOSIDES | |
dc.subject | 7-DEAZA-2'-DEOXYGUANOSINES | |
dc.subject | Biotechnology & Applied Microbiology | |
dc.subject | CHAINS | |
dc.subject | DNA polymerases | |
dc.subject | ion-exchange HPLC | |
dc.subject | LENGTH | |
dc.subject | NUCLEIC-ACIDS | |
dc.subject | nucleoside triphosphates | |
dc.subject | oligodeoxynucleotides | |
dc.subject | OLIGONUCLEOTIDES | |
dc.subject | STABILITY | |
dc.title | Fluorescent DNA: the development of 7-deazapurine nucleoside triphosphates applicable for sequencing at the single molecule level | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/S0168-1656(00)00418-1 | |
dc.identifier.isi | ISI:000167948900008 | |
dc.description.volume | 86 | |
dc.description.issue | 3, SI | |
dc.description.startpage | 269 | |
dc.description.endpage | 279 | |
dc.identifier.eissn | 18734863 | |
dc.publisher.place | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS | |
dcterms.isPartOf.abbreviation | J. Biotechnol. | |
crisitem.author.dept | Institut für Chemie neuer Materialien | - |
crisitem.author.deptid | institute11 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | RoHe783 | - |
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geprüft am 07.06.2024