COMPARISON OF THE SEQUENCES OF THE NAGE OPERONS FROM KLEBSIELLA-PNEUMONIAE AND ESCHERICHIA-COLI K12 - ENHANCED VARIABILITY OF THE ENZYME IIN-ACETYLGLUCOSAMINE IN REGIONS CONNECTING FUNCTIONAL DOMAINS

Autor(en): VOGLER, AP
LENGELER, JW
Stichwörter: BACILLUS-SUBTILIS; BACTERIAL PHOSPHOENOLPYRUVATE; Biochemistry & Molecular Biology; CARBOHYDRATE PHOSPHOTRANSFERASE SYSTEM; DNA; ENTERIC BACTERIA; ENZYME IINAG; FUNCTIONAL DOMAINS; Genetics & Heredity; HOMOLOGIES; LINKER; MANNOSE PERMEASE; N-ACETYLGLUCOSAMINE; NAGE OPERON; REGULATORY PROTEINS; TRANSPORT PROTEIN
Erscheinungsdatum: 1991
Herausgeber: SPRINGER VERLAG
Journal: MOLECULAR & GENERAL GENETICS
Volumen: 230
Ausgabe: 1-2
Startseite: 270
Seitenende: 276
Zusammenfassung: 
The nagE operon, encoding the enzyme II specific for N-acetylglucosamine (EII(Nag)), and adjacent DNA from the chromosome of Klebsiella pneumoniae were sequenced and compared with the corresponding sequence from Escherichia coli K12. The deduced EII(Nag) sequences differ in 72 out of 651 amino acids, the K. pneumoniae sequence being three residues longer. The amino acid differences were distributed unevenly, and were most frequent in regions connecting the three functional domains of the protein. In the nagE-nagB intergenic region, two promoter, two operator, and one CAP consensus sequence with regulatory functions were highly conserved. The nag structural genes from both species were very similar (83% DNA similarity; 89% amino acid similarity) except for frequent AT to GC exchanges in the wobble base of codons in K. pneumoniae DNA relative to the E. coli DNA.
ISSN: 00268925
DOI: 10.1007/BF00290677

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