THE SUGAR-SPECIFIC OUTER-MEMBRANE CHANNEL SCRY CONTAINS FUNCTIONAL-CHARACTERISTICS OF GENERAL DIFFUSION PORES AND SUBSTRATE-SPECIFIC PORINS

Autor(en): SCHULEIN, K
SCHMID, K
BENZL, R
Stichwörter: BINDING-PROTEIN; Biochemistry & Molecular Biology; ESCHERICHIA-COLI K-12; GRAM-NEGATIVE BACTERIA; LAMBDA-RECEPTOR; LIPID BILAYER-MEMBRANES; MALTOSE TRANSPORT; MATRIX PROTEIN PORIN; Microbiology; MOLECULAR-BASIS; PSEUDOMONAS-AERUGINOSA; SALMONELLA-TYPHIMURIUM
Erscheinungsdatum: 1991
Herausgeber: BLACKWELL SCIENCE LTD
Journal: MOLECULAR MICROBIOLOGY
Volumen: 5
Ausgabe: 9
Startseite: 2233
Seitenende: 2241
Zusammenfassung: 
Escherichia coli K-12 strain PS1-28-37 carries the multicopy plasmid pPSO28-37 containing a DNA fragment coding for two of the proteins that enable bacteria to utilize sucrose as sole carbon source. One of the different gene products of the plasmid is the outer membrane protein, ScrY. This protein was isolated and purified by chromatography across a gel filtration column. Reconstitution experiments with lipid bilayer membrane demonstrated that ScrY formed ion-permeable channels with properties very similar to those of general diffusion pores of enteric bacteria. The presence of sugars in the aqueous phase led to a dose-dependent block of ion transport through the channel, like the situation found with LamB (maltoporin) of Escherichia coli and Salmonella typhimurium. The binding constants of a variety of different sugars were determined. The stability constant for malto-oligosaccharide binding increased with increasing numbers of glucose residues. Disaccharides generally had a larger binding constant than monosaccharides. The binding of different sugars to ScrY and LamB of E. coli is discussed with respect to the kinetics of sugar movement through the channel.
ISSN: 0950382X
DOI: 10.1111/j.1365-2958.1991.tb02153.x

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