Monofunctional Stealth Nanoparticle for Unbiased Single Molecule Tracking Inside Living Cells
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Lisse, Domenik | |
dc.contributor.author | Richter, Christian P. | |
dc.contributor.author | Drees, Christoph | |
dc.contributor.author | Birkholz, Oliver | |
dc.contributor.author | You, Changjiang | |
dc.contributor.author | Rampazzo, Enrico | |
dc.contributor.author | Piehler, Jacob | |
dc.date.accessioned | 2021-12-23T16:09:57Z | - |
dc.date.available | 2021-12-23T16:09:57Z | - |
dc.date.issued | 2014 | |
dc.identifier.issn | 15306984 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/9072 | - |
dc.description.abstract | On the basis of a protein cage scaffold, we have systematically explored intracellular application of nanoparticles for single molecule studies and discovered that recognition by the autophagy machinery plays a key role for rapid metabolism in the cytosol. Intracellular stealth nanoparticles were achieved by heavy surface PEGylation. By combination with a generic approach for nanoparticle monofunctionalization, efficient labeling of intracellular proteins with high fidelity was accomplished, allowing unbiased long-term tracking of proteins in the outer mitochondrial membrane. | |
dc.description.sponsorship | DFGGerman Research Foundation (DFG)European Commission [SFB 944]; HFSPHuman Frontier Science Program [RGP0005/2007]; We thank Gabriele Hikade and Hella Kenneweg for technical support, Stefan Walter for mass spectrometry analyses and Sergej Korneev for synthesis of the Halo Tag ligand. The cDNA for LC3B was kindly provided by Sascha Martens, Vienna. We also thank him and Fulvio Reggiori, Utrecht, for instructive discussions. This project was supported by funding from the DFG (SFB 944) and by the HFSP (RGP0005/2007). | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | NANO LETTERS | |
dc.subject | AUTOPHAGY | |
dc.subject | BINDING | |
dc.subject | BIOMOLECULES | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | Chemistry, Physical | |
dc.subject | cytosol | |
dc.subject | DENSITY | |
dc.subject | DIFFUSION | |
dc.subject | IMPACT | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | MEMBRANES | |
dc.subject | mitochondria | |
dc.subject | monofunctionalization | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.subject | Physics, Condensed Matter | |
dc.subject | PROTEIN CORONA | |
dc.subject | PROVIDE | |
dc.subject | QUANTUM DOTS | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | single particle tracking | |
dc.subject | Stealth nanoparticle | |
dc.title | Monofunctional Stealth Nanoparticle for Unbiased Single Molecule Tracking Inside Living Cells | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/nl500637a | |
dc.identifier.isi | ISI:000334572400078 | |
dc.description.volume | 14 | |
dc.description.issue | 4 | |
dc.description.startpage | 2189 | |
dc.description.endpage | 2195 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.contributor.orcid | 0000-0001-6551-3219 | |
dc.contributor.orcid | 0000-0003-2290-859X | |
dc.contributor.researcherid | L-3901-2014 | |
dc.identifier.eissn | 15306992 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | Nano Lett. | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | YoCh745 | - |
crisitem.author.netid | PiJa938 | - |
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geprüft am 12.05.2024