Photosystem II of green plants: Topology of core pigments and redox cofactors as inferred from electrochromic difference spectra

DC FieldValueLanguage
dc.contributor.authorMulkidjanian, AY
dc.contributor.authorCherepanov, DA
dc.contributor.authorHaumann, M
dc.contributor.authorJunge, W
dc.date.accessioned2021-12-23T16:10:10Z-
dc.date.available2021-12-23T16:10:10Z-
dc.date.issued1996
dc.identifier.issn00062960
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/9197-
dc.description.abstractThree electrochromic difference spectra induced by the deposition of (1) a negative charge on the primary quinone acceptor, Q(A), (2) a positive charge on (or near) Tyr161 of the D1 subunit (Y-Z), and (3) a positive charge on the manganese cluster were determined at room temperature in photosystem II (PSII) core particles from pea. They were deconvoluted into Gaussian components by Powell's numerical optimization procedure. All three spectra were fitted by four components, which we assigned to the Q,. absorption bands of two chlorophyll a molecules of the primary donor P, the accessory chlorophyll a, and the pheophytin a molecules on the D1 subunit. On the basis of the electrochromic properties of chlorins and our data, we suggest an arrangement of pigments and redox cofactors in PSII that differs from current structural models, which have been shaped like the reaction centers (RC) of purple bacteria. Our model is compatible with sequence data, with the spectroscopic and electrochemical properties of chlorophyll a and pheophytin a, and with the extremely positive redox potential of water oxidation. We conclude the following: (1) P is formed from two orthogonally oriented chlorophyll a molecules that peak at 681 and 677 nm. (2) The accessory chlorophyll a on D1 is oriented perpendicular to the membrane, with ring V pointing to Q(A). It is presumably attached to His118 of D1. (3) The mutual arrangement of pheophytin a on the D1 subunit and Q(A) differs from that of their counterparts in bacterial RC. (4) The manganese cluster is located out of the axis that is formed by Y-Z (Tyr161 of D1), P, and Y-D (Tyr161 of D2).
dc.language.isoen
dc.publisherAMER CHEMICAL SOC
dc.relation.ispartofBIOCHEMISTRY
dc.subjectBACTERIAL PHOTOSYNTHESIS
dc.subjectBiochemistry & Molecular Biology
dc.subjectCHLOROFLEXUS-AURANTIACUS
dc.subjectOXYGEN-EVOLVING SYSTEM
dc.subjectPEA CHLOROPLAST DNA
dc.subjectPHOTOSYNTHETIC REACTION CENTER
dc.subjectPRIMARY ELECTRON-DONOR
dc.subjectREACTION CENTER COMPLEX
dc.subjectRHODOBACTER-SPHAEROIDES
dc.subjectRHODOPSEUDOMONAS-SPHAEROIDES
dc.subjectTYROSINE RADICALS
dc.titlePhotosystem II of green plants: Topology of core pigments and redox cofactors as inferred from electrochromic difference spectra
dc.typereview
dc.identifier.doi10.1021/bi9513057
dc.identifier.isiISI:A1996TZ11800036
dc.description.volume35
dc.description.issue9
dc.description.startpage3093
dc.description.endpage3107
dc.contributor.orcid0000-0001-6286-4638
dc.contributor.orcid0000-0001-5844-3064
dc.contributor.researcheridR-8391-2016
dc.contributor.researcheridA-7087-2013
dc.contributor.researcheridJ-8086-2013
dc.contributor.researcheridAAH-3608-2021
dc.publisher.place1155 16TH ST, NW, WASHINGTON, DC 20036 USA
dcterms.isPartOf.abbreviationBiochemistry
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidJuWo587-
Show simple item record

Google ScholarTM

Check

Altmetric