Preparation of Arabidopsis mesophyll protoplasts with high rates of photosynthesis

DC FieldValueLanguage
dc.contributor.authorRiazunnisa, Khateef
dc.contributor.authorPadmavathi, Lolla
dc.contributor.authorScheibe, Renate
dc.contributor.authorRaghavendra, Agepati S.
dc.date.accessioned2021-12-23T16:10:18Z-
dc.date.available2021-12-23T16:10:18Z-
dc.date.issued2007
dc.identifier.issn00319317
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/9264-
dc.description.abstractA simple and quick method is described for rapid isolation of metabolically active mesophyll protoplasts from leaves of Arabidopsis thaliana. The optimal composition of the digestion medium, period of digestion and stability of protoplast preparation were examined. A large number of protoplasts could be prepared within an hour. The isolated protoplasts were intact, stable and metabolically very active, as indicated by their high rates of photosynthetic oxygen evolution. The important factors during the preparation of protoplasts are short time of digestion, composition of medium, use of nylon nets for filtration, centrifugation at low speed and use of pH 7.0 for storage. The highest rate of photosynthesis obtained in these experiments was 130 /- 4 mu mol O-2 evolved mg(-1) Chl h(-1), at 1 mM sodium bicarbonate and at a light intensity of 600 mu E m(-2) s(-1). The present technique of isolation can be very useful for making Arabidopsis protoplasts for studies on not only metabolic processes, such as photosynthesis, but also metabolomics, proteomics and genomics.
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofPHYSIOLOGIA PLANTARUM
dc.subjectBARLEY
dc.subjectCELL-DEATH
dc.subjectCHANNELS
dc.subjectCHLOROPLASTS
dc.subjectGUARD
dc.subjectLIGHT
dc.subjectPlant Sciences
dc.subjectRESPIRATION
dc.subjectSYSTEM
dc.subjectTHALIANA
dc.subjectTRANSPORTER
dc.titlePreparation of Arabidopsis mesophyll protoplasts with high rates of photosynthesis
dc.typejournal article
dc.identifier.doi10.1111/j.1399-3054.2007.00867.x
dc.identifier.isiISI:000244944800002
dc.description.volume129
dc.description.issue4
dc.description.startpage679
dc.description.endpage686
dc.contributor.orcid0000-0002-7376-4947
dc.contributor.orcid0000-0003-2245-0451
dc.contributor.researcheridAAR-8316-2020
dc.contributor.researcheridC-2166-2011
dc.identifier.eissn13993054
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationPhysiol. Plant.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-6140-6181-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidScRe288-
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