A quantitative approach to catabolite repression in Escherichia coli

Autor(en): Bettenbrock, K
Fischer, S
Kremling, A
Jahreis, K
Sauter, T
Gilles, ED
Stichwörter: Biochemistry & Molecular Biology; CAMP RECEPTOR PROTEIN; DIAUXIC GROWTH; GLUCOSE-TRANSPORTER; INDUCER EXCLUSION; MAJOR GLUCOSE; METABOLIC REACTION NETWORKS; MLC; PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE; PTS; SIGNAL-TRANSDUCTION
Erscheinungsdatum: 2006
Herausgeber: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Volumen: 281
Ausgabe: 5
Startseite: 2578
Seitenende: 2584
Zusammenfassung: 
A dynamic mathematical model was developed to describe the uptake of various carbohydrates ( glucose, lactose, glycerol, sucrose, and galactose) in Escherichia coli. For validation a number of isogenic strains with defined mutations were used. By considering metabolic reactions as well as signal transduction processes influencing the relevant pathways, we were able to describe quantitatively the phenomenon of catabolite repression in E. coli. We verified model predictions by measuring time courses of several extra-and intracellular components such as glycolytic intermediates, EII-A(Crr) phosphorylation level, both LacZ and PtsG concentrations, and total cAMP concentrations under various growth conditions. The entire data base consists of 18 experiments performed with nine different strains. The model describes the expression of 17 key enzymes, 38 enzymatic reactions, and the dynamic behavior of more than 50 metabolites. The different phenomena affecting the phosphorylation level of EIIA(Crr), the key regulation molecule for inducer exclusion and catabolite repression in enteric bacteria, can now be explained quantitatively.
ISSN: 00219258
DOI: 10.1074/jbc.M508090200

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