ROXY1 and ROXY2, two Arabidopsis glutaredoxin genes, are required for anther development

DC FieldValueLanguage
dc.contributor.authorXing, Shuping
dc.contributor.authorZachgo, Sabine
dc.date.accessioned2021-12-23T16:10:39Z-
dc.date.available2021-12-23T16:10:39Z-
dc.date.issued2008
dc.identifier.issn09607412
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/9332-
dc.description.abstractGlutaredoxins (GRXs) are small oxidoreductases that are involved in various cellular processes and play a crucial role in responses to oxidative stress. Three GRX subgroups exist in plants, and GRXs with active sites of the CPYC and CGFS types are common to pro- and eukaryotes. In contrast, GRXs with the CC type motif have so far only been identified in land plants. Here, we report that the two CC-type GRXs ROXY1 and ROXY2 together control anther development in Arabidopsis thaliana. Single roxy1 and roxy2 mutants are fertile and produce normal anthers. However, roxy1 roxy2 double mutants are sterile and do not produce pollen. Strikingly, abaxial and adaxial anther lobe differentiation are differently affected, with early lobe differentiation being defective in the adaxial lobes, whereas later steps during pollen mother cell differentiation are disrupted in the abaxial lobes. Expression studies show that ROXY1 and ROXY2 are expressed with overlapping patterns during anther development. Lack of ROXY1 and ROXY2 function affects a large number of anther genes at the transcriptional level. Genetic and RT-PCR data imply that ROXY1/2 function downstream of the early-acting anther gene SPOROCYTELESS/NOZZLE and upstream of DYSFUNCTIONAL TAPETUM1, controlling tapetum development. Mutagenesis of a conserved glutathione-binding glycine in the ROXY1 protein indicates that CC-type GRXs need to interact with glutathione to catalyze essential biosynthetic reactions. Analysis of these two novel anther genes indicates that redox regulation, as well as participating in plant stress defense mechanisms, might play a major role in the control of male gametogenesis.
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofPLANT JOURNAL
dc.subjectanther development
dc.subjectArabidopsis
dc.subjectENCODES
dc.subjectFAMILY
dc.subjectFLOWER DEVELOPMENT
dc.subjectgene expression
dc.subjectglutaredoxin
dc.subjectGLUTATHIONE
dc.subjectMUTANT
dc.subjectPlant Sciences
dc.subjectPROTEIN
dc.subjectREDOX REGULATION
dc.subjectSPOROGENESIS
dc.subjecttapetum
dc.subjectTAPETUM DEVELOPMENT
dc.subjectTHALIANA
dc.titleROXY1 and ROXY2, two Arabidopsis glutaredoxin genes, are required for anther development
dc.typejournal article
dc.identifier.doi10.1111/j.1365-313X.2007.03375.x
dc.identifier.isiISI:000253484600008
dc.description.volume53
dc.description.issue5
dc.description.startpage790
dc.description.endpage801
dc.identifier.eissn1365313X
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationPlant J.
dcterms.oaStatusGreen Submitted
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidZaSa518-
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