D-Altritol nucleic acids (ANA): Hybridisation properties, stability, and initial structural analysis

DC FieldValueLanguage
dc.contributor.authorAllart, B
dc.contributor.authorKhan, K
dc.contributor.authorRosemeyer, H
dc.contributor.authorSchepers, G
dc.contributor.authorHendrix, C
dc.contributor.authorRothenbacher, K
dc.contributor.authorSeela, F
dc.contributor.authorVan Aerschot, A
dc.contributor.authorHerdewijn, P
dc.date.accessioned2021-12-23T16:10:41Z-
dc.date.available2021-12-23T16:10:41Z-
dc.date.issued1999
dc.identifier.issn09476539
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/9343-
dc.description.abstractOligonucleotides composed of a phosphorylated D-altritol backbone with nucleobases introduced in the 2'-position, hybridise strongly and sequence-selectively with RNA in an antiparallel way. The order of hybridisation strength is: dsANA > ANA:RNA > ANA:DNA. Complexes between ANA and RNA dr DNA are more stable than between HNA and natural oligonucleotides. The dsANA hybrid is extremely stable. When compared with an identical dsDNA sequence, the Delta T-m per modification is 10.2 degrees C for a hexamer. CD spectral analysis indicates that ANA complexes are very similar to the A-form dsRNA duplex. ANA are stable in alkaline medium up to pH 12 and, likewise, are not degraded in human serum.
dc.language.isoen
dc.publisherWILEY-V C H VERLAG GMBH
dc.relation.ispartofCHEMISTRY-A EUROPEAN JOURNAL
dc.subject1',5'-ANHYDROHEXITOL OLIGONUCLEOTIDES
dc.subjectaltritol nucleic acids
dc.subjectantisense agents
dc.subjectBASE-PAIRING PROPERTIES
dc.subjectChemistry
dc.subjectChemistry, Multidisciplinary
dc.subjectDNA
dc.subjecthexitol nucleic acids
dc.subjectMOIETY
dc.subjectNUCLEOSIDES
dc.subjectoligonucleotides
dc.subjectOLIGORIBONUCLEOTIDES
dc.subjectRECOGNITION
dc.subjectthermal stability data
dc.titleD-Altritol nucleic acids (ANA): Hybridisation properties, stability, and initial structural analysis
dc.typejournal article
dc.identifier.doi10.1002/(SICI)1521-3765(19990802)5:8<2424::AID-CHEM2424>3.0.CO;2-W
dc.identifier.isiISI:000081866100025
dc.description.volume5
dc.description.issue8
dc.description.startpage2424
dc.description.endpage2431
dc.contributor.orcid0000-0003-3528-8588
dc.contributor.orcid0000-0003-3589-8503
dc.publisher.placeMUHLENSTRASSE 33-34, D-13187 BERLIN, GERMANY
dcterms.isPartOf.abbreviationChem.-Eur. J.
crisitem.author.deptInstitut für Chemie neuer Materialien-
crisitem.author.deptidinstitute11-
crisitem.author.parentorgFB 05 - Biologie/Chemie-
crisitem.author.grandparentorgUniversität Osnabrück-
crisitem.author.netidRoHe783-
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