Individual Interactions of the b Subunits within the Stator of the Escherichia coli ATP Synthase

Autor(en): Brandt, Karsten
Maiwald, Sarah
Herkenhoff-Hesselmann, Brigitte
Gnirss, Kerstin
Greie, Joerg-Christian
Dunn, Stanley D.
Deckers-Hebestreit, Gabriele 
Stichwörter: 2ND STALK; ALPHA-SUBUNIT; Biochemistry & Molecular Biology; DELTA SUBUNITS; DIMERIZATION DOMAIN; EPSILON-SUBUNIT; F1F0-ATP SYNTHASE; H+-ATPASE; PERIPHERAL STALK; PROTON-TRANSLOCATING ATPASE; TERMINAL REGION
Erscheinungsdatum: 2013
Herausgeber: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Volumen: 288
Ausgabe: 34
Startseite: 24465
Seitenende: 24479
Zusammenfassung: 
FOF1 ATP synthases are rotary nanomotors that couple proton translocation across biological membranes to the synthesis/hydrolysis of ATP. During catalysis, the peripheral stalk, composed of two b subunits and subunit delta in Escherichia coli, counteracts the torque generated by the rotation of the central stalk. Here we characterize individual interactions of the b subunits within the stator by use of monoclonal antibodies and nearest neighbor analyses via intersubunit disulfide bond formation. Antibody binding studies revealed that the C-terminal region of one of the two b subunits is principally involved in the binding of subunit delta, whereas the other one is accessible to antibody binding without impact on the function of FOF1. Individually substituted cysteine pairs suitable for disulfide cross-linking between the b subunits and the other stator subunits (b-alpha, b-beta, b-delta, and b-a) were screened and combined with each other to discriminate between the two b subunits (i.e. b(I) and b(II)). The results show the b dimer to be located at a non-catalytic alpha/beta cleft, with b(I) close to subunit alpha, whereas b(II) is proximal to subunit beta. Furthermore, b(I) can be linked to subunit delta as well as to subunit a. Among the subcomplexes formed were a-b(I)-alpha, b(II)-beta, alpha-b(I)-b(II)-beta, and a-b(I)-delta. Taken together, the data obtained define the different positions of the two b subunits at a non-catalytic interface and imply that each b subunit has a different role in generating stability within the stator. We suggest that b(I) is functionally related to the single b subunit present in mitochondrial ATP synthase.
DOI: 10.1074/jbc.M113.465633

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