Chitin is a necessary component to maintain the barrier function of the peritrophic matrix in the insect midgut

DC FieldValueLanguage
dc.contributor.authorKelkenberg, Marco
dc.contributor.authorOdman-Naresh, Jothini
dc.contributor.authorMuthukrishnan, Subbaratnam
dc.contributor.authorMerzendorfer, Hans
dc.date.accessioned2021-12-23T16:11:01Z-
dc.date.available2021-12-23T16:11:01Z-
dc.date.issued2015
dc.identifier.issn09651748
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/9495-
dc.description.abstractIn most insects, the peritrophic matrix (PM) partitions the midgut into different digestive compartments, and functions as a protective barrier against abrasive particles and microbial infections. In a previous study we demonstrated that certain PM proteins are essential in maintaining the PM's barrier function and establishing a gradient of PM permeability from the anterior to the posterior part of the midgut which facilitates digestion (Agrawal et al., 2014). In this study, we focused on the effects of a reduction in chitin content on PM permeability in larvae of the red flour beetle, Tribolium castaneum. Oral administration of the chitin synthesis inhibitor diflubenzuron (DFB) only partially reduced chitin content of the larval PM even at high concentrations. We observed no nutritional effects, as larval growth was unaffected and neutral lipids were not depleted from the fat body. However, the metamorphic molt was disrupted and the insects died at the pharate pupal stage, presumably due to DFB's effect on cuticle formation. RNAi to knock-down expression of the gene encoding chitin synthase 2 in T. castaneum (TcCHS-2) caused a complete loss of chitin in the PM. Larval growth was significantly reduced, and the fat body was depleted of neutral lipids. In situ PM permeability assays monitoring the distribution of FITC dextrans after DFB exposure or RNAi for TcCHS-2 revealed that PM permeability was increased in both cases. RNAi for TcCHS-2, however, led to a higher permeation of the PM by FITC dextrans than DFB treatment even at high doses. Similar effects were observed when the chitin content was reduced by feeding DFB to adult yellow fever mosquitos, Aedes aegypti. We demonstrate that the presence of chitin is necessary for maintaining the PM's barrier function in insects. It seems that the insecticidal effects of DFB are mediated by the disruption of cuticle synthesis during the metamorphic molt rather than by interfering with larval nutrition. However, as DFB clearly affects PM permeability, it may be suitable to increase the efficiency of pesticides targeting the midgut. (C) 2014 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipNSF GrantNational Science Foundation (NSF) [IOS-1022227]; DFGGerman Research Foundation (DFG)European Commission [Me2210/3-1, SFB944-P6]; Government of the State of Lower Saxonia, Germany; This work was supported by NSF Grant IOS-1022227 to S. Muthukrishnan and DFG Grants Me2210/3-1 and SFB944-P6 to H. Merzendorfer. Marco Kelkenberg was largely supported by a Lichtenberg fellowship awarded by the Government of the State of Lower Saxonia, Germany.
dc.language.isoen
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofINSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
dc.subjectAedes aegypti
dc.subjectAEDES-AEGYPTI
dc.subjectBiochemistry & Molecular Biology
dc.subjectChitin
dc.subjectDELTA-ENDOTOXIN
dc.subjectDIFLUBENZURON
dc.subjectEntomology
dc.subjectFALL ARMYWORM
dc.subjectMEMBRANE
dc.subjectPeritrophic matrix
dc.subjectPermeability
dc.subjectRED FLOUR BEETLE
dc.subjectRNA interference
dc.subjectSYNTHASE GENES
dc.subjectSYNTHESIS INHIBITORS
dc.subjectTribolium castaneum
dc.subjectTRIBOLIUM-CASTANEUM
dc.subjectWHEAT-GERM-AGGLUTININ
dc.titleChitin is a necessary component to maintain the barrier function of the peritrophic matrix in the insect midgut
dc.typejournal article
dc.identifier.doi10.1016/j.ibmb.2014.11.005
dc.identifier.isiISI:000348626700003
dc.description.volume56
dc.description.startpage21
dc.description.endpage28
dc.identifier.eissn18790240
dc.publisher.placeTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
dcterms.isPartOf.abbreviationInsect Biochem. Mol. Biol.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidMeHa731-
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