A reference scale of cucurbit[7]uril binding affinities
Autor(en): | Alnajjar, Mohammad A. Nau, Werner M. Hennig, Andreas |
Stichwörter: | BERBERINE; Chemistry; Chemistry, Organic; COMPLEXATION; CUCURBITURIL; FLUORESCENT DYE DISPLACEMENT; GUESTS; HOST; ISOTHERMAL TITRATION CALORIMETRY; PEPTIDES; TANDEM ASSAYS; URIL | Erscheinungsdatum: | 2021 | Herausgeber: | ROYAL SOC CHEMISTRY | Journal: | ORGANIC & BIOMOLECULAR CHEMISTRY | Volumen: | 19 | Ausgabe: | 39 | Startseite: | 8521 | Seitenende: | 8529 | Zusammenfassung: | The accurate determination of ultra-high binding affinities in supramolecular host-guest chemistry is a challenging endeavour because direct binding titrations are generally limited to affinities <10(6) M-1 due to sensitivity constraints of common titration methods. To determine higher affinities, competitive titrations are usually performed, in which one compound with a well established binding affinity serves as a reference. Herein, we propose a reference scale for such competitive titrations with the host cucurbit[7]uril (CB7) comprising binding affinities in the range from 10(3) to 10(15) M-1. The suggested reference compounds are commercially available and will aid in the future determination of CB7 binding affinities for stimuli-responsive host-guest systems. |
ISSN: | 14770520 | DOI: | 10.1039/d1ob01304a |
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