DC Element | Wert | Sprache |
dc.contributor.author | Broeermann, Andreas | |
dc.contributor.author | Steinhoff, Heinz-Juergen | |
dc.contributor.author | Schluecker, Sebastian | |
dc.date.accessioned | 2021-12-23T16:11:13Z | - |
dc.date.available | 2021-12-23T16:11:13Z | - |
dc.date.issued | 2014 | |
dc.identifier.issn | 00222860 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/9591 | - |
dc.description.abstract | The site-specific pH is an experimental probe for assessing models of structural folding and function of a protein as well as protein protein and protein ligand interactions. It can be determined by various techniques such as NMR, FT-IR, fluorescence and EPR spectroscopy. The latter require the use of external labels, i.e., employ pH-dependent dyes and spin labels, respectively. In this contribution, we outline an approach to a label-free and site-specific method for determining the local pH using deep ultraviolet resonance Raman (UVRR) spectroscopic fingerprints of the aromatic amino acids histidine and tyrosine in combination with a robust algorithm that determines the pH value using three UVRR reference spectra and without prior knowledge of the pK(a). (C) 2014 Elsevier B.V. All rights reserved. | |
dc.description.sponsorship | German Research Foundation (DFG)German Research Foundation (DFG) [INST 190/129-1]; University of Osnabruck; Financial support by the German Research Foundation (DFG, INST 190/129-1) and the University of Osnabruck is acknowleged. We thank Dr. Johann Klare for providing Fig. 1. | |
dc.language.iso | en | |
dc.publisher | ELSEVIER SCIENCE BV | |
dc.relation.ispartof | JOURNAL OF MOLECULAR STRUCTURE | |
dc.subject | AMINO-ACIDS | |
dc.subject | Aromatic amino acids | |
dc.subject | BIOPHYSICAL CHEMISTRY | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Physical | |
dc.subject | DYNAMICS | |
dc.subject | H-BONDS | |
dc.subject | LIVING CELLS | |
dc.subject | Microenvironment | |
dc.subject | PEPTIDE RECOGNITION | |
dc.subject | RESIDUES | |
dc.subject | Resonance Raman spectroscopy | |
dc.subject | SENSORY RHODOPSIN II | |
dc.subject | SPECTROSCOPY | |
dc.subject | WATER | |
dc.title | Towards label-free and site-specific probing of the local pH in proteins: pH-dependent deep UV Raman spectra of histidine and tyrosine | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/j.molstruc.2014.03.053 | |
dc.identifier.isi | ISI:000340301800013 | |
dc.description.volume | 1073 | |
dc.description.issue | SI | |
dc.description.startpage | 77 | |
dc.description.endpage | 81 | |
dc.contributor.orcid | 0000-0002-5888-0157 | |
dc.contributor.researcherid | H-3791-2014 | |
dc.identifier.eissn | 18728014 | |
dc.publisher.place | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS | |
dcterms.isPartOf.abbreviation | J. Mol. Struct. | |
crisitem.author.dept | FB 04 - Physik | - |
crisitem.author.deptid | fb04 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | StHe633 | - |