Engineered Upconversion Nanoparticles for Resolving Protein Interactions inside Living Cells

DC FieldValueLanguage
dc.contributor.authorDrees, Christoph
dc.contributor.authorRaj, Athira Naduviledathu
dc.contributor.authorKurre, Rainer
dc.contributor.authorBusch, Karin B.
dc.contributor.authorHaase, Markus
dc.contributor.authorPiehler, Jacob
dc.date.accessioned2021-12-23T16:11:23Z-
dc.date.available2021-12-23T16:11:23Z-
dc.date.issued2016
dc.identifier.issn14337851
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/9674-
dc.description.abstractUpconversion nanoparticles (UCNPs) convert near-infrared into visible light at much lower excitation densities than those used in classic two-photon absorption microscopy. Here, we engineered <50nm UCNPs for application as efficient lanthanide resonance energy transfer (LRET) donors inside living cells. By optimizing the dopant concentrations and the core-shell structure for higher excitation densities, we observed enhanced UCNP emission as well as strongly increased sensitized acceptor fluorescence. For the application of these UCNPs in complex biological environments, we developed a biocompatible surface coating functionalized with a nanobody recognizing green fluorescent protein (GFP). Thus, rapid and specific targeting to GFP-tagged fusion proteins in the mitochondrial outer membrane and detection of protein interactions by LRET in living cells was achieved.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 944]; European Commission (FET-open) [686841-MAGNEURON]; We thank Gabriele Hikade, Hella Kenneweg, Junel Sotolongo Bellon, and Wladislaw Kohl for technical support as well as Domenik Lisse, Christian Richter, Jorg Nordmann, Thorben Rinkel, and Changjiang You for helpful advice. This project was supported by the Deutsche Forschungsgemeinschaft (SFB 944) and by the European Commission (FET-open 686841-MAGNEURON).
dc.language.isoen
dc.publisherWILEY-V C H VERLAG GMBH
dc.relation.ispartofANGEWANDTE CHEMIE-INTERNATIONAL EDITION
dc.subjectbiosensors
dc.subjectChemistry
dc.subjectChemistry, Multidisciplinary
dc.subjectEMERGING APPLICATIONS
dc.subjectGOLD NANORODS
dc.subjectLRET
dc.subjectLUMINESCENCE NANOPLATFORM
dc.subjectNANOCRYSTALS
dc.subjectnanoparticles
dc.subjectOPTICAL-PROPERTIES
dc.subjectPHOTODYNAMIC THERAPY
dc.subjectPHOTON-UPCONVERTING NANOPARTICLES
dc.subjectRESONANCE ENERGY-TRANSFER
dc.subjectSHELL THICKNESS
dc.subjectTOM complexes
dc.subjectULTRASENSITIVE DETECTION
dc.subjectupconversion
dc.titleEngineered Upconversion Nanoparticles for Resolving Protein Interactions inside Living Cells
dc.typejournal article
dc.identifier.doi10.1002/anie.201603028
dc.identifier.isiISI:000383748900068
dc.description.volume55
dc.description.issue38
dc.description.startpage11668
dc.description.endpage11672
dc.contributor.orcid0000-0003-0525-0191
dc.contributor.orcid0000-0002-9686-8810
dc.contributor.researcheridAAM-8374-2021
dc.contributor.researcheridABH-8594-2020
dc.identifier.eissn15213773
dc.publisher.placePOSTFACH 101161, 69451 WEINHEIM, GERMANY
dcterms.isPartOf.abbreviationAngew. Chem.-Int. Edit.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptInstitut für Chemie neuer Materialien-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.deptidinstitute11-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-6872-6567-
crisitem.author.orcid0000-0002-9686-8810-
crisitem.author.orcid0000-0002-2143-2270-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgFB 05 - Biologie/Chemie-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.grandparentorgUniversität Osnabrück-
crisitem.author.netidKuRa617-
crisitem.author.netidHaMa954-
crisitem.author.netidPiJa938-
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