A systematic study of the cell wall composition of Kluyveromyces lactis

DC FieldValueLanguage
dc.contributor.authorBackhaus, Katja
dc.contributor.authorHeilmann, Clemens J.
dc.contributor.authorSorgo, Alice G.
dc.contributor.authorPurschke, Guenter
dc.contributor.authorde Koster, Chris G.
dc.contributor.authorKlis, Frans M.
dc.contributor.authorHeinisch, Juergen J.
dc.date.accessioned2021-12-23T16:11:37Z-
dc.date.available2021-12-23T16:11:37Z-
dc.date.issued2010
dc.identifier.issn0749503X
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/9794-
dc.description.abstractIn many ascomycetous yeasts, the cell wall is composed of two main types of macromolecules: (a) polysaccharides, with a high content of beta-1,6- and beta-1,3-linked glucan chains and minor amounts of chitin; and (b) cell wall proteins of different types. Synthesis and maintenance of these macromolecules respond to environmental changes, which are sensed by the cell wall integrity (CWI) signal transduction pathway. We here present a first systematic analysis of the cell wall composition of the milk yeast, Kluyveromyces lactis. Electron microscopic analyses revealed that exponentially growing cells of K. lactis supplied with glucose as a carbon source have a wall thickness of 64 nm, as compared to 105 nm when growing on 3% ethanol. Despite their increased wall thickness, ethanol-grown cells were more sensitive to the presence of zymolyase in the growth medium. Mass spectrometric analysis identified 22 covalently linked cell wall proteins, including 19 GPI-modified proteins and two Pir wall proteins. Importantly, the composition of the cell wall glycoproteome depended on carbon source and growth phase. Our results clearly illustrate the dynamic nature of the cell wall of K. lactis and provide a firm base for studying its regulation. Copyright (C) 2010 John Wiley & Sons, Ltd.
dc.description.sponsorshipEUEuropean Commission [FP7-214004-2 FINSysB]; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 431]; We are grateful to Bernadette Sander-Turgut for technical assistance and Rosaura Rodicio for critical reading of the manuscript. F.M.K. acknowledges financial support by the EU Programme, Grant No. FP7-214004-2 FINSysB. The work in Osnabruck was funded by the Deutsche Forschungsgemeinschaft (Grant No. SFB 431, to J.J.H).
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofYEAST
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiotechnology & Applied Microbiology
dc.subjectcell wall proteins
dc.subjectCHITIN
dc.subjectELECTRON-MICROSCOPY
dc.subjectglucan
dc.subjectINTEGRITY
dc.subjectmannan
dc.subjectMAP KINASE
dc.subjectMETABOLISM
dc.subjectMicrobiology
dc.subjectMOLECULAR-GENETICS
dc.subjectMycology
dc.subjectOXIDATIVE STRESS-RESPONSE
dc.subjectproteomics
dc.subjectSACCHAROMYCES-CEREVISIAE
dc.subjectSENSOR
dc.subjectYEAST-CELL
dc.titleA systematic study of the cell wall composition of Kluyveromyces lactis
dc.typejournal article
dc.identifier.doi10.1002/yea.1781
dc.identifier.isiISI:000281227000020
dc.description.volume27
dc.description.issue8, SI
dc.description.startpage647
dc.description.endpage660
dc.contributor.orcid0000-0002-2572-3390
dc.contributor.orcid0000-0003-2872-2796
dc.contributor.researcheridG-3801-2017
dc.contributor.researcheridAAU-9660-2021
dc.identifier.eissn10970061
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationYeast
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-2572-3390-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidPuGu978-
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