A systematic study of the cell wall composition of Kluyveromyces lactis

Autor(en): Backhaus, Katja
Heilmann, Clemens J.
Sorgo, Alice G.
Purschke, Guenter 
de Koster, Chris G.
Klis, Frans M.
Heinisch, Juergen J.
Stichwörter: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; cell wall proteins; CHITIN; ELECTRON-MICROSCOPY; glucan; INTEGRITY; mannan; MAP KINASE; METABOLISM; Microbiology; MOLECULAR-GENETICS; Mycology; OXIDATIVE STRESS-RESPONSE; proteomics; SACCHAROMYCES-CEREVISIAE; SENSOR; YEAST-CELL
Erscheinungsdatum: 2010
Herausgeber: WILEY
Journal: YEAST
Volumen: 27
Ausgabe: 8, SI
Startseite: 647
Seitenende: 660
In many ascomycetous yeasts, the cell wall is composed of two main types of macromolecules: (a) polysaccharides, with a high content of beta-1,6- and beta-1,3-linked glucan chains and minor amounts of chitin; and (b) cell wall proteins of different types. Synthesis and maintenance of these macromolecules respond to environmental changes, which are sensed by the cell wall integrity (CWI) signal transduction pathway. We here present a first systematic analysis of the cell wall composition of the milk yeast, Kluyveromyces lactis. Electron microscopic analyses revealed that exponentially growing cells of K. lactis supplied with glucose as a carbon source have a wall thickness of 64 nm, as compared to 105 nm when growing on 3% ethanol. Despite their increased wall thickness, ethanol-grown cells were more sensitive to the presence of zymolyase in the growth medium. Mass spectrometric analysis identified 22 covalently linked cell wall proteins, including 19 GPI-modified proteins and two Pir wall proteins. Importantly, the composition of the cell wall glycoproteome depended on carbon source and growth phase. Our results clearly illustrate the dynamic nature of the cell wall of K. lactis and provide a firm base for studying its regulation. Copyright (C) 2010 John Wiley & Sons, Ltd.
ISSN: 0749503X
DOI: 10.1002/yea.1781

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