PURIFICATION AND CHARACTERIZATION OF FERREDOXIN FROM PEPTOSTREPTOCOCCUS-PRODUCTUS (STRAIN MARBURG)
Autor(en): | REUBELT, U WOHLFARTH, G SCHMID, R DIEKERT, G |
Stichwörter: | ACETATE FORMATION; ACETOBACTERIUM-WOODII; ACETOGENIC BACTERIA; CARBON MONOXIDE DEHYDROGENASE; CELL-SUSPENSIONS; CLOSTRIDIUM-THERMOACETICUM; CO2; FERREDOXIN; HOMOACETOGENIC BACTERIA; IRON; Microbiology; PEPTOSTREPTOCOCCUS-PRODUCTUS (STRAIN MARBURG); RUBREDOXIN | Erscheinungsdatum: | 1991 | Herausgeber: | SPRINGER VERLAG | Enthalten in: | ARCHIVES OF MICROBIOLOGY | Band: | 156 | Ausgabe: | 5 | Startseite: | 422 | Seitenende: | 426 | Zusammenfassung: | Ferredoxin was purified to apparent homogeneity from cell extracts of the homoacetogen Peptostreptococcus productus (strain Marburg). The yield was 70-mu-g ferredoxin per g wet cells of P. productus. The UV-vis spectrum exhibited characteristics of a typical clostridial ferredoxin spectrum with a molar extinction coefficient epsilon-385 of approximately 30 000 M-1 cm-1 and an A385/A280 ratio of 0.76. The molecular weight M(r) was near 5700 as calculated from the amino acid composition. The protein contained per mol 9.9 mol iron, 8.2 mol acid-labile sulfide, and near 7 mol cysteine indicating the presence of two 4 Fe/4 S clusters. The redox potential was determined to be -410 mV. The purified ferredoxin was reduced with carbon monoxide by the carbon monoxide dehydrogenase from crude extracts and by the partially enriched enzyme of P. productus. |
ISSN: | 03028933 | DOI: | 10.1007/BF00248721 |
Zur Langanzeige
Seitenaufrufe
1
Letzte Woche
0
0
Letzter Monat
0
0
geprüft am 06.06.2024