PURIFICATION AND CHARACTERIZATION OF FERREDOXIN FROM PEPTOSTREPTOCOCCUS-PRODUCTUS (STRAIN MARBURG)

Autor(en): REUBELT, U
WOHLFARTH, G
SCHMID, R
DIEKERT, G
Stichwörter: ACETATE FORMATION; ACETOBACTERIUM-WOODII; ACETOGENIC BACTERIA; CARBON MONOXIDE DEHYDROGENASE; CELL-SUSPENSIONS; CLOSTRIDIUM-THERMOACETICUM; CO2; FERREDOXIN; HOMOACETOGENIC BACTERIA; IRON; Microbiology; PEPTOSTREPTOCOCCUS-PRODUCTUS (STRAIN MARBURG); RUBREDOXIN
Erscheinungsdatum: 1991
Herausgeber: SPRINGER VERLAG
Journal: ARCHIVES OF MICROBIOLOGY
Volumen: 156
Ausgabe: 5
Startseite: 422
Seitenende: 426
Zusammenfassung: 
Ferredoxin was purified to apparent homogeneity from cell extracts of the homoacetogen Peptostreptococcus productus (strain Marburg). The yield was 70-mu-g ferredoxin per g wet cells of P. productus. The UV-vis spectrum exhibited characteristics of a typical clostridial ferredoxin spectrum with a molar extinction coefficient epsilon-385 of approximately 30 000 M-1 cm-1 and an A385/A280 ratio of 0.76. The molecular weight M(r) was near 5700 as calculated from the amino acid composition. The protein contained per mol 9.9 mol iron, 8.2 mol acid-labile sulfide, and near 7 mol cysteine indicating the presence of two 4 Fe/4 S clusters. The redox potential was determined to be -410 mV. The purified ferredoxin was reduced with carbon monoxide by the carbon monoxide dehydrogenase from crude extracts and by the partially enriched enzyme of P. productus.
ISSN: 03028933
DOI: 10.1007/BF00248721

Show full item record

Page view(s)

1
Last Week
0
Last month
0
checked on Mar 1, 2024

Google ScholarTM

Check

Altmetric