The bHLH transcription factor hand is required for proper wing heart formation in Drosophila

Autor(en): Toegel, Markus
Meyer, Heiko
Lehmacher, Christine
Heinisch, Juergen J.
Pass, Guenther
Paululat, Achim 
Stichwörter: Adult myogenesis; Armadillo; BRANCHIAL ARCH; CARDIAC MORPHOGENESIS; CARDIOGENESIS; CELLS; Developmental Biology; DHAND; DIFFERENTIATION; Dystroglycan; GENE; HEMATOPOIESIS; MUSCLE; Muscle attachment; PROTEINS; Spectrin; Wing maturation
Erscheinungsdatum: 2013
Volumen: 381
Ausgabe: 2
Startseite: 446
Seitenende: 459
The Hand basic helix-loop-helix transcription factors play an important role in the specification and patterning of various tissues in vertebrates and invertebrates. Here, we have investigated the function of Hand in the development of the Drosophila wing hearts which consist of somatic muscle cells as well as a mesodermally derived epithelium. We found that Hand is essential in both tissues for proper organ formation. Loss of Hand leads to a reduced number of cells in the mature organ and loss of wing heart functionality. In wing heart muscles Hand is required for the correct positioning of attachment sites, the parallel alignment of muscle cells, and the proper orientation of myofibrils. At the protein level, alpha-Spectrin and Dystroglycan are misdistributed suggesting a defect in the costameric network. Hand is also required for proper differentiation of the wing heart epithelium. Additionally, the handC-GFP reporter line is not active in the mutant suggesting an autoregulatory role of Hand in wing hearts. Finally, in a candidate-based RNAi mediated knock-down approach we identified Daughterless and Nautilus as potential dimerization partners of Hand in wing hearts. (C) 2013 Elsevier Inc. All rights reserved.
ISSN: 00121606
DOI: 10.1016/j.ydbio.2013.05.027

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