The study of structural accessibility of free thiol groups in human low-density lipoproteins

Autor(en): Kveder, M
Krisko, A
Pifat, G
Steinhoff, HJ
Stichwörter: APOLIPOPROTEIN-B; B-100; BACTERIORHODOPSIN; Biochemistry & Molecular Biology; Biophysics; Cell Biology; CONFORMATIONAL-CHANGES; denaturation of apoB; DOMAINS; EPR; HIGH-FIELD EPR; LDL; OXIDATION; SEQUENCE; SULFHYDRYL; UREA
Erscheinungsdatum: 2003
Herausgeber: ELSEVIER SCIENCE BV
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Volumen: 1631
Ausgabe: 3
Startseite: 239
Seitenende: 245
Zusammenfassung: 
The experimental evidence for the apolipoprotein B 100 (apoB) domain structuring in low-density lipoprotein (LDL) was investigated focusing on the accessibility of free thiol groups. Three different spectroscopic methods were combined with the biochemical perturbations of LDL particle. The spectrophotometric method was adapted for LDL and the exposure of free thiols was analyzed in the native LDL and LDL exposed to sequential denaturation. The results indicate that 24-h denaturation does not expose all free thiols in LDL. Using thiol-specific spin labeling and electron paramagnetic resonance spectroscopy (EPR), different populations of labeled thiols were resolved. The comparison of the EPR spectra of native LDL and LDL with selectively blocked thiol groups revealed significant difference in the respective hyperfine splittings. The phenomenon can arise due to different polarity and/or mobility of the nitroxides in the microenvironments of spin label binding sites of these two LDL samples. The results indicate that nine thiol groups in apoB are distributed in different domains of LDL: two are more exposed, two are buried deeply in the lipid matrix of the particle and the rest are located in hydrophobic parts of this extremely complex protein-lipid assembly. These observations provide experimental support for the emerging theoretical models of apoB. (C) 2003 Elsevier Science B.V. All rights reserved.
ISSN: 13881981
DOI: 10.1016/S1388-1981(03)00022-2

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