Mapping and identification of Corynebacterium glutamicum proteins by two-dimensional gel electrophoresis and microsequencing

DC FieldValueLanguage
dc.contributor.authorHermann, T
dc.contributor.authorWersch, G
dc.contributor.authorUhlemann, EM
dc.contributor.authorSchmid, R
dc.contributor.authorBurkovski, A
dc.date.accessioned2021-12-23T16:11:50Z-
dc.date.available2021-12-23T16:11:50Z-
dc.date.issued1998
dc.identifier.issn01730835
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/9912-
dc.description.abstractAs a prerequisite for proteome analyses of Corynebacterium glutamicum separation of the cytoplasm and the membrane fraction was optimized and two-dimensional (2-D) gel electrophoresis was established. The resulting 2-D protein maps revealed over 1000 silver-stained protein spots separated by isoelectric point and molecular mass for cytoplasmic proteins and approximately 700 silver-stained spots for proteins of the membrane fraction. Proposing a mean size of 1 kbp per gene the complete C. glutamicum genome of 3 Mbp encodes 3000 different proteins; more than half of these can be located using the maps which are presently available. In this study 10 proteins were identified by N-terminal microsequencing, namely the 35 kDa antigen, antigen 84, ATP synthase subunits alpha, gamma and delta, cysteine synthase, elongation factor G and Ts, enolase, and rotamase. For seven sequences, corresponding proteins could not be identified. Additionally, two proteins were specifically detected by immunoblotting, a corynebacterial porin and the cytoplasmic protein threonine dehydratase. The methods and 2-D maps established in this study will be the basis for comparative studies of protein expression and a detailed proteome analysis of C. glutamicum.
dc.language.isoen
dc.publisherWILEY-V C H VERLAG GMBH
dc.relation.ispartofELECTROPHORESIS
dc.subject2-DIMENSIONAL ELECTROPHORESIS
dc.subjectACID
dc.subjectBACILLUS-SUBTILIS
dc.subjectBiochemical Research Methods
dc.subjectBiochemistry & Molecular Biology
dc.subjectChemistry
dc.subjectChemistry, Analytical
dc.subjectCorynebacterium glutamicum
dc.subjectESCHERICHIA-COLI
dc.subjectIMMOBILIZED PH GRADIENTS
dc.subjectmicrosequencing
dc.subjectPHYSIOLOGY
dc.subjectprotein mapping
dc.subjectproteomics
dc.subjectSTATE
dc.subjecttwo-dimensional polyacrylamide gel electrophoresis
dc.titleMapping and identification of Corynebacterium glutamicum proteins by two-dimensional gel electrophoresis and microsequencing
dc.typejournal article
dc.identifier.doi10.1002/elps.1150191827
dc.identifier.isiISI:000078204800026
dc.description.volume19
dc.description.issue18
dc.description.startpage3217
dc.description.endpage3221
dc.contributor.orcid0000-0003-1896-4521
dc.publisher.placeMUHLENSTRASSE 33-34, D-13187 BERLIN, GERMANY
dcterms.isPartOf.abbreviationElectrophoresis
Show simple item record

Page view(s)

2
Last Week
0
Last month
0
checked on Apr 19, 2024

Google ScholarTM

Check

Altmetric