Characterization of a proteasome alpha-chain from Giardia lamblia

Autor(en): Emmerlich, V
Scholze, H
Gillin, FD
Bakker-Grunwald, T
Stichwörter: GENE; Parasitology; RNA
Erscheinungsdatum: 2001
Herausgeber: SPRINGER
Volumen: 87
Ausgabe: 2
Startseite: 112
Seitenende: 115
To begin to characterize the components of the 20S proteasome of Giardia lamblia, we have cloned a genomic sequence encoding an alpha -chain (type alpha3/C9, predicted size 244 amino acid residues). Southern analysis indicated that a single gene codes for this protein, and a Northern blot exhibited a single signal at 850 nt. An antiserum against a C-terminal fragment of the alpha -chain expressed in Escherichia coli reacted with a single protein band of M-r 27,000 that was present at constant levels in trophozoites and encysting cells. On a 2D blot of the purified 20S proteasome, we identified the crossreacting component as a single protein of IEP 6.0, in agreement with the IEP predicted by the coding sequence. Our data confirm that the G. lamblia 20S proteasome is typically eukaryotic in containing a set of diverged alpha -subunits.
ISSN: 09320113
DOI: 10.1007/s004360000332

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