Characterization of a proteasome alpha-chain from Giardia lamblia
Autor(en): | Emmerlich, V Scholze, H Gillin, FD Bakker-Grunwald, T |
Stichwörter: | GENE; Parasitology; RNA | Erscheinungsdatum: | 2001 | Herausgeber: | SPRINGER | Journal: | PARASITOLOGY RESEARCH | Volumen: | 87 | Ausgabe: | 2 | Startseite: | 112 | Seitenende: | 115 | Zusammenfassung: | To begin to characterize the components of the 20S proteasome of Giardia lamblia, we have cloned a genomic sequence encoding an alpha -chain (type alpha3/C9, predicted size 244 amino acid residues). Southern analysis indicated that a single gene codes for this protein, and a Northern blot exhibited a single signal at 850 nt. An antiserum against a C-terminal fragment of the alpha -chain expressed in Escherichia coli reacted with a single protein band of M-r 27,000 that was present at constant levels in trophozoites and encysting cells. On a 2D blot of the purified 20S proteasome, we identified the crossreacting component as a single protein of IEP 6.0, in agreement with the IEP predicted by the coding sequence. Our data confirm that the G. lamblia 20S proteasome is typically eukaryotic in containing a set of diverged alpha -subunits. |
ISSN: | 09320113 | DOI: | 10.1007/s004360000332 |
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geprüft am 21.05.2024