1',5'-anhydrohexitol oligonucleotides: Hybridisation and strand displacement with oligoribonucleotides, interaction with RNase H and HIV reverse transcriptase

Autor(en): Hendrix, C
Rosemeyer, H 
DeBouvere, B
VanAerschot, A
Seela, F
Herdewijn, P
Stichwörter: Chemistry; Chemistry, Multidisciplinary; duplex; gel mobility; nucleic acids; NUCLEIC-ACIDS; NUCLEOSIDES; oligonucleotides; strand displacement
Erscheinungsdatum: 1997
Herausgeber: WILEY-V C H VERLAG GMBH
Journal: CHEMISTRY-A EUROPEAN JOURNAL
Volumen: 3
Ausgabe: 9
Startseite: 1513
Seitenende: 1520
Zusammenfassung: 
Hexitol nucleic acids (HNAs) with four natural bases form stable and sequence-selective duplexes with RNA. This was investigated by T-m determinations and gel shift experiments. The CD spectra of an HNA-RNA duplex show similarities with the CD spectra of the A-form of dsRNA. Single-stranded HNAs are able to induce strand displacement in a double-stranded RNA sequence. An HNA-RNA duplex is a poor substrate for RNase H, and can inhibit the RNase H-mediated cleavage of a natural DNA-RNA substrate. The HNA-RNA hybrid enhances the activity of HIV reverse transcriptase.
ISSN: 09476539
DOI: 10.1002/chem.19970030920

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