COMPARATIVE-ANALYSIS OF SODIUM-DEPENDENT L-GLUTAMATE TRANSPORT OF SYNAPTOSOMAL AND ASTROGLIAL MEMBRANE-VESICLES FROM MOUSE CORTEX

DC FieldValueLanguage
dc.contributor.authorRAUEN, T
dc.contributor.authorJESERICH, G
dc.contributor.authorDANBOLT, NC
dc.contributor.authorKANNER, BI
dc.date.accessioned2021-12-23T16:14:00Z-
dc.date.available2021-12-23T16:14:00Z-
dc.date.issued1992
dc.identifier.issn00145793
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/10857-
dc.description.abstractUptake of [H-3]L-glutamate into membrane vesicles prepared from either mouse cortical astrocyte cultures or synaptosomes was found to be an electrogenic sodium- and potassium-dependent transport process with saturable uptake kinetics. Pharmacological differences were revealed by using a variety of substrate analogues. L-trans-PDC inhibited the synaptosomal glutamate transport 2-4-fold stronger than the astroglial uptake. The substrate analogues DL-threo-beta-hydroxy-aspartate, DL-aspartate-beta-hydroxamate, L-aspartate and D-aspartate inhibited glutamate transport of astroglial and neuronal membrane vesicles in a distinctive manner, whereas D-glutamate, quisqualate and dihydrokainate had no effect in either case. Immunoblotting and immunocytochemical labeling with antibodies against the rat brain glutamate transporter revealed the selective reaction of a band at about 75 kDa mol. wt. and a specific pattern of astrocyte immunostaining.
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofFEBS LETTERS
dc.subjectACTIVE-TRANSPORT
dc.subjectASTROGLIAL MEMBRANE VESICLE
dc.subjectBINDING
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCell Biology
dc.subjectGAMMA-AMINOBUTYRIC-ACID
dc.subjectGLIAL CELLS
dc.subjectGLUTAMATE ANALOG
dc.subjectHIGH-AFFINITY UPTAKE
dc.subjectIMMUNOBLOTTING
dc.subjectIMMUNOCYTOCHEMISTRY
dc.subjectL-ASPARTATE
dc.subjectL-GLUTAMATE TRANSPORT
dc.subjectMETABOLISM
dc.subjectPROTEINS
dc.subjectRAT-BRAIN SLICES
dc.subjectSYNAPTOSOME
dc.subjectUPTAKE SYSTEMS
dc.titleCOMPARATIVE-ANALYSIS OF SODIUM-DEPENDENT L-GLUTAMATE TRANSPORT OF SYNAPTOSOMAL AND ASTROGLIAL MEMBRANE-VESICLES FROM MOUSE CORTEX
dc.typejournal article
dc.identifier.doi10.1016/0014-5793(92)81401-7
dc.identifier.isiISI:A1992JV99800005
dc.description.volume312
dc.description.issue1
dc.description.startpage15
dc.description.endpage20
dc.contributor.orcid0000-0003-4227-4520
dc.contributor.orcid0000-0001-9699-182X
dc.contributor.researcheridA-1907-2014
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dcterms.isPartOf.abbreviationFEBS Lett.
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