COMPARATIVE-ANALYSIS OF SODIUM-DEPENDENT L-GLUTAMATE TRANSPORT OF SYNAPTOSOMAL AND ASTROGLIAL MEMBRANE-VESICLES FROM MOUSE CORTEX
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | RAUEN, T | |
dc.contributor.author | JESERICH, G | |
dc.contributor.author | DANBOLT, NC | |
dc.contributor.author | KANNER, BI | |
dc.date.accessioned | 2021-12-23T16:14:00Z | - |
dc.date.available | 2021-12-23T16:14:00Z | - |
dc.date.issued | 1992 | |
dc.identifier.issn | 00145793 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/10857 | - |
dc.description.abstract | Uptake of [H-3]L-glutamate into membrane vesicles prepared from either mouse cortical astrocyte cultures or synaptosomes was found to be an electrogenic sodium- and potassium-dependent transport process with saturable uptake kinetics. Pharmacological differences were revealed by using a variety of substrate analogues. L-trans-PDC inhibited the synaptosomal glutamate transport 2-4-fold stronger than the astroglial uptake. The substrate analogues DL-threo-beta-hydroxy-aspartate, DL-aspartate-beta-hydroxamate, L-aspartate and D-aspartate inhibited glutamate transport of astroglial and neuronal membrane vesicles in a distinctive manner, whereas D-glutamate, quisqualate and dihydrokainate had no effect in either case. Immunoblotting and immunocytochemical labeling with antibodies against the rat brain glutamate transporter revealed the selective reaction of a band at about 75 kDa mol. wt. and a specific pattern of astrocyte immunostaining. | |
dc.language.iso | en | |
dc.publisher | ELSEVIER SCIENCE BV | |
dc.relation.ispartof | FEBS LETTERS | |
dc.subject | ACTIVE-TRANSPORT | |
dc.subject | ASTROGLIAL MEMBRANE VESICLE | |
dc.subject | BINDING | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Biophysics | |
dc.subject | Cell Biology | |
dc.subject | GAMMA-AMINOBUTYRIC-ACID | |
dc.subject | GLIAL CELLS | |
dc.subject | GLUTAMATE ANALOG | |
dc.subject | HIGH-AFFINITY UPTAKE | |
dc.subject | IMMUNOBLOTTING | |
dc.subject | IMMUNOCYTOCHEMISTRY | |
dc.subject | L-ASPARTATE | |
dc.subject | L-GLUTAMATE TRANSPORT | |
dc.subject | METABOLISM | |
dc.subject | PROTEINS | |
dc.subject | RAT-BRAIN SLICES | |
dc.subject | SYNAPTOSOME | |
dc.subject | UPTAKE SYSTEMS | |
dc.title | COMPARATIVE-ANALYSIS OF SODIUM-DEPENDENT L-GLUTAMATE TRANSPORT OF SYNAPTOSOMAL AND ASTROGLIAL MEMBRANE-VESICLES FROM MOUSE CORTEX | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/0014-5793(92)81401-7 | |
dc.identifier.isi | ISI:A1992JV99800005 | |
dc.description.volume | 312 | |
dc.description.issue | 1 | |
dc.description.startpage | 15 | |
dc.description.endpage | 20 | |
dc.contributor.orcid | 0000-0003-4227-4520 | |
dc.contributor.orcid | 0000-0001-9699-182X | |
dc.contributor.researcherid | A-1907-2014 | |
dc.publisher.place | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS | |
dcterms.isPartOf.abbreviation | FEBS Lett. |
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