The single kinin receptor signals to separate and independent physiological pathways in Malpighian tubules of the yellow fever mosquito

DC FieldValueLanguage
dc.contributor.authorSchepel, Stephen A.
dc.contributor.authorFox, Andrew J.
dc.contributor.authorMiyauchi, Jeremy T.
dc.contributor.authorSou, Tiffany
dc.contributor.authorYang, Jason D.
dc.contributor.authorLau, Kenneth
dc.contributor.authorBlum, Austin W.
dc.contributor.authorNicholson, Linda K.
dc.contributor.authorTiburcy, Felix
dc.contributor.authorNachman, Ronald J.
dc.contributor.authorPiermarini, Peter M.
dc.contributor.authorBeyenbach, Klaus W.
dc.date.accessioned2021-12-23T16:15:54Z-
dc.date.available2021-12-23T16:15:54Z-
dc.date.issued2010
dc.identifier.issn03636119
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/11636-
dc.description.abstractSchepel SA, Fox AJ, Miyauchi JT, Sou T, Yang JD, Lau K, Blum AW, Nicholson LK, Tiburcy F, Nachman RJ, Piermarini PM, Beyenbach KW. The single kinin receptor signals to separate and independent physiological pathways in Malpighian tubules of the yellow fever mosquito. Am J Physiol Regul Integr Comp Physiol 299: R612-R622, 2010. First published June 10, 2010; doi: 10.1152/ajpregu.00068.2010.-In the past, we have used the kinins of the cockroach Leucophaea (the leucokinins) to evaluate the mechanism of diuretic action of kinin peptides in Malpighian tubules of the yellow fever mosquito Aedes aegypti. Now using the kinins of Aedes (the aedeskinins), we have found that in isolated Aedes Malpighian tubules all three aedeskinins (1 mu M) significantly 1) increased the rate of fluid secretion ((V) over dot(S)), 2) hyperpolarized the basolateral membrane voltage (V-bl), and 3) decreased the input resistance (R-in) of principal cells, consistent with the known increase in the Cl- conductance of the paracellular pathway in Aedes Malpighian tubules. Aedeskinin-III, studied in further detail, significantly increased (V) over dot(S) with an EC50 of 1.5 x 10(-8) M. In parallel, the Na+ concentration in secreted fluid significantly decreased, and the K+ concentration significantly increased. The concentration of Cl- remained unchanged. While the three aedeskinins triggered effects on V-bl, R-in, and (V) over dot(S) synthetic kinin analogs, which contain modifications of the COOH-terminal amide pentapeptide core sequence critical for biological activity, displayed variable effects. For example, kinin analog 1578 significantly stimulated (V) over dot(S) but had no effect on V-bl and R-in, whereas kinin analog 1708 had no effect on (V) over dot(S) but significantly affected V-bl and R-in. These observations suggest separate signaling pathways activated by kinins. One triggers the electrophysiological response, and the other triggers fluid secretion. It remains to be determined whether the two signaling pathways emanate from a single kinin receptor via agonist-directed signaling or from a differentially glycosylated receptor. Occasionally, Malpighian tubules did not exhibit a detectable response to natural and synthetic kinins. Hypothetically, the expression of the kinin receptor may depend on developmental, nutritional, and/or reproductive signals.
dc.description.sponsorshipNational Science FoundationNational Science Foundation (NSF) [IBN-0078058]; U.S. Department of Agriculture/Department of Defense, Deployed War Fighter Protection InitiativeUnited States Department of DefenseUnited States Department of Agriculture (USDA) [0500-32000-001-01R]; NATIONAL INSTITUTE ON AGINGUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute on Aging (NIA) [R01AG029385] Funding Source: NIH RePORTER; The National Science Foundation Award IBN-0078058 made this work possible in the Beyenbach laboratory, and the U.S. Department of Agriculture/Department of Defense, Deployed War Fighter Protection Initiative No. 0500-32000-001-01R supported work in the Nachman laboratory.
dc.language.isoen
dc.publisherAMER PHYSIOLOGICAL SOC
dc.relation.ispartofAMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
dc.subjectAedes aegypti
dc.subjectAEDES-AEGYPTI DIPTERA
dc.subjectaedeskinin
dc.subjectalpha- and beta-analogs of insect kinins
dc.subjectBIOLOGICAL-ACTIVITY
dc.subjectDIRECTED TRAFFICKING
dc.subjectDIURETIC PEPTIDES
dc.subjectDROSOPHILA-MELANOGASTER
dc.subjectEFFECTOR PATHWAY
dc.subjectfunctionally elective agonists
dc.subjectG protein-coupled kinin receptor
dc.subjectGLYCOSYLATION SITES
dc.subjectinput resistance
dc.subjectLEUCOKININ RECEPTOR
dc.subjectmembrane voltage
dc.subjectPhysiology
dc.subjectprincipal cells
dc.subjectPROTEIN-COUPLED RECEPTOR
dc.subjectRamsay fluid secretion assay
dc.subjectTICK BOOPHILUS-MICROPLUS
dc.titleThe single kinin receptor signals to separate and independent physiological pathways in Malpighian tubules of the yellow fever mosquito
dc.typejournal article
dc.identifier.doi10.1152/ajpregu.00068.2010
dc.identifier.isiISI:000280569800024
dc.description.volume299
dc.description.issue2
dc.description.startpageR612-R622
dc.contributor.orcid0000-0002-2234-2306
dc.contributor.orcid0000-0003-1020-5774
dc.contributor.researcheridAAO-5666-2021
dc.identifier.eissn15221490
dc.publisher.place9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA
dcterms.isPartOf.abbreviationAm. J. Physiol.-Regul. Integr. Comp. Physiol.
dcterms.oaStatusGreen Published
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidTiFe418-
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