Time-resolved methods in Biophysics. 1. A novel pump and probe surface-enhanced resonance Raman approach for studying biological photoreceptors

Autor(en): Naumann, Hendrik
Klare, Johann P.
Engelhard, Martin
Hildebrandt, Peter
Murgida, Daniel H.
Stichwörter: ABSORPTION; ACTIVE-SITE STRUCTURE; Biochemistry & Molecular Biology; Biophysics; Chemistry; Chemistry, Physical; CYTOCHROME-C; ELECTRON-TRANSFER; OXIDASE; PHARAONIS; PHOTOCYCLE; REDOX PROCESSES; SENSORY RHODOPSIN-II; SPECTROSCOPY
Erscheinungsdatum: 2006
Herausgeber: SPRINGERNATURE
Journal: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
Volumen: 5
Ausgabe: 12
Startseite: 1103
Seitenende: 1108
Zusammenfassung: 
This article describes a method, based on surface-enhanced resonance Raman (SERR) spectroscopy, for studying the reaction dynamics of photoreceptors immobilized on metal electrodes. Time resolution and fresh sample conditions are achieved by synchronizing the rotational and translational motion of a novel kinematic electrode with the duration and time delay between the pump and probe events. The power and sensitivity of the method is illustrated by studying the photocycle of the sensory photoreceptor NpSRII and its sensitivity to the applied electric field. The results are compared with time-resolved resonance Raman measurements in solution.
ISSN: 1474905X
DOI: 10.1039/b607807a

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