Molecular architecture of Manduca sexta midgut V-1 ATPase visualized by electron microscopy
Autor(en): | Radermacher, M Ruiz, T Harvey, WR Wieczorek, H Gruber, G |
Stichwörter: | Biochemistry & Molecular Biology; BIOLOGICAL MACROMOLECULES; Biophysics; Cell Biology; electron microscopy; ESCHERICHIA-COLI; image processing; IMAGES; Manduca sexta; MEMBRANE; PARTICLES; PURIFICATION; SUBUNIT; TOBACCO HORNWORM MIDGUT; V-1 ATPase; VACUOLAR-TYPE ATPASE | Erscheinungsdatum: | 1999 | Herausgeber: | ELSEVIER SCIENCE BV | Journal: | FEBS LETTERS | Volumen: | 453 | Ausgabe: | 3 | Startseite: | 383 | Seitenende: | 386 | Zusammenfassung: | The structure of the V-1 ATPase from the tobacco hornworm Manduca sexta has been determined from electron micrographs of isolated, negatively stained specimens. The resulting images clearly show a pseudohexagonal arrangement of six equal-sized protein densities, presumably representing the three copies each of subunits A and B, which comprise the headpiece of the enzyme. A seventh density could be observed either centrally or asymmetrically to the hexamer, The maximum diameter of the V-1 complex in the hexagonal projection is 13 nm with each of the six peripheral densities being 3-4 nm in diameter. (C) 1999 Federation of European Biochemical Societies. |
ISSN: | 00145793 | DOI: | 10.1016/S0014-5793(99)00739-5 |
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geprüft am 17.05.2024