Covalent Monofunctionalization of Peptide-Coated Quantum Dots for Single-Molecule Assays

Autor(en): Clarke, Samuel
Pinaud, Fabien
Beutel, Oliver
You, Changjiang 
Piehler, Jacob 
Dahan, Maxime
Stichwörter: cell imaging; Chemistry; Chemistry, Multidisciplinary; Chemistry, Physical; fluorescent probes; LIVE CELLS; Materials Science; Materials Science, Multidisciplinary; MEMBRANE; MONOVALENT; NANOPARTICLES; Nanoscience & Nanotechnology; particle tracking; Physics; Physics, Applied; Physics, Condensed Matter; PROTEINS; Quantum dots; RECEPTORS; Science & Technology - Other Topics; SEMICONDUCTOR NANOCRYSTALS; single molecules; SIZE; STABILITY; TRACKING
Erscheinungsdatum: 2010
Herausgeber: AMER CHEMICAL SOC
Journal: NANO LETTERS
Volumen: 10
Ausgabe: 6
Startseite: 2147
Seitenende: 2154
Zusammenfassung: 
Fluorescent probes For biological imaging of single molecules (SM) have many stringent design requirements. In the case of quantum dot (QD) probes, it remains a challenge to control their functional properties with high precision. Here, we describe the simple preparation of QDs with reduced size and monovalency. Our approach combines a peptide surface coating, stable covalent conjugation of targeting units and purification by gel electrophoresis. We precisely characterize these probes by ensemble and SM techniques and apply them to tracking individual proteins in living cells.
ISSN: 15306984
DOI: 10.1021/nl100825n

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