Covalent Monofunctionalization of Peptide-Coated Quantum Dots for Single-Molecule Assays
DC Element | Wert | Sprache |
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dc.contributor.author | Clarke, Samuel | |
dc.contributor.author | Pinaud, Fabien | |
dc.contributor.author | Beutel, Oliver | |
dc.contributor.author | You, Changjiang | |
dc.contributor.author | Piehler, Jacob | |
dc.contributor.author | Dahan, Maxime | |
dc.date.accessioned | 2021-12-23T16:20:18Z | - |
dc.date.available | 2021-12-23T16:20:18Z | - |
dc.date.issued | 2010 | |
dc.identifier.issn | 15306984 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/13404 | - |
dc.description.abstract | Fluorescent probes For biological imaging of single molecules (SM) have many stringent design requirements. In the case of quantum dot (QD) probes, it remains a challenge to control their functional properties with high precision. Here, we describe the simple preparation of QDs with reduced size and monovalency. Our approach combines a peptide surface coating, stable covalent conjugation of targeting units and purification by gel electrophoresis. We precisely characterize these probes by ensemble and SM techniques and apply them to tracking individual proteins in living cells. | |
dc.description.sponsorship | Universite Pierre et Marie Curie; Fondation pour la Recherche MedicaleFondation pour la Recherche Medicale; Marie Curie-Intra-European FellowshipEuropean Commission [MEIF-CT-2006-040210]; EMBOEuropean Molecular Biology Organization (EMBO); Fondation pour la Recherche Medicale, Agence Nationale pour la RechercheFondation pour la Recherche MedicaleFrench National Research Agency (ANR) [ANR-05-PNANO-045]; Human Frontier Science ProgramHuman Frontier Science Program [RGP0005/2007]; centre C'Nano Ile de France; Region Ile-de-FranceRegion Ile-de-France [SESAME 2000 E 1435]; C'Nano Ile-de-FrancRegion Ile-de-France; We thank Thomas Le Saux (FCS), Eric Larquet (EM), Geraldine Gouzer (transfection), and Mariya Batch (data analysis) for assistance during various experiments and Alice Ting of MIT for providing plasmids for expression of AP-CFP-TM, BirA-ER, and the streptavidin variants. S.C. acknowledges support from postdoctoral fellowships through Universite Pierre et Marie Curie and the Fondation pour la Recherche Medicale. F.P. acknowledges financial support from a Marie Curie-Intra-European Fellowship under contract number MEIF-CT-2006-040210, and an EMBO long-term fellowship. M.D. acknowledges support from the Fondation pour la Recherche Medicale, Agence Nationale pour la Recherche (Grant ANR-05-PNANO-045), the Human Frontier Science Program (Grant RGP0005/2007) and the centre C'Nano Ile de France. We are grateful to Region Ile-de-France for convention SESAME 2000 E 1435 and to C'Nano Ile-de-France for supporting cryo-EM at IMPMC. | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | NANO LETTERS | |
dc.subject | cell imaging | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | Chemistry, Physical | |
dc.subject | fluorescent probes | |
dc.subject | LIVE CELLS | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | MEMBRANE | |
dc.subject | MONOVALENT | |
dc.subject | NANOPARTICLES | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | particle tracking | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.subject | Physics, Condensed Matter | |
dc.subject | PROTEINS | |
dc.subject | Quantum dots | |
dc.subject | RECEPTORS | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | SEMICONDUCTOR NANOCRYSTALS | |
dc.subject | single molecules | |
dc.subject | SIZE | |
dc.subject | STABILITY | |
dc.subject | TRACKING | |
dc.title | Covalent Monofunctionalization of Peptide-Coated Quantum Dots for Single-Molecule Assays | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/nl100825n | |
dc.identifier.isi | ISI:000278449200030 | |
dc.description.volume | 10 | |
dc.description.issue | 6 | |
dc.description.startpage | 2147 | |
dc.description.endpage | 2154 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.contributor.orcid | 0000-0002-4943-2491 | |
dc.contributor.orcid | 0000-0002-4272-3616 | |
dc.contributor.researcherid | F-2306-2010 | |
dc.contributor.researcherid | L-3901-2014 | |
dc.contributor.researcherid | F-1740-2010 | |
dc.identifier.eissn | 15306992 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | Nano Lett. | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.orcid | 0000-0002-2143-2270 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | YoCh745 | - |
crisitem.author.netid | PiJa938 | - |
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