Structure and regulation of the dnaA promoter region in three Streptomyces species

Autor(en): Jakimowicz, D
Majka, J
Lis, B
Konopa, G
Wegrzyn, G
Messer, W
Schrempf, H 
Zakrzewska-Czerwinska, J
Stichwörter: AUTO-REGULATION; autoregulation; BINDING; Biochemistry & Molecular Biology; CHROMOSOME; DnaA box; DnaA protein; ELEMENTS; ESCHERICHIA-COLI; GENE; Genetics & Heredity; INITIATOR PROTEIN DNAA; promoter; REPLICATION; SEQUENCE; TRANSCRIPTIONAL ACTIVATION
Erscheinungsdatum: 2000
Herausgeber: SPRINGER
Journal: MOLECULAR AND GENERAL GENETICS
Volumen: 262
Ausgabe: 6
Startseite: 1093
Seitenende: 1102
Zusammenfassung: 
The regulatory region of the Streptomyces dnaA gene comprises a single promoter and two DnaA boxes that are located upstream of the promoter. Comparative analysis of the dnaA promoter region from S. chrysomallus, S. lividans and S. reticuli revealed that the location, spacing and orientation of the DnaA boxes are conserved. In vitro studies demonstrated that efficient binding of the Streptomyces DnaA protein to DNA requires the presence of two DnaA boxes. In vivo analysis of dnaA promoter mutants deleted for one or both DnaA boxes indicated that the dnaA gene is autoregulated. However, the degree of derepression observed is relatively modest.
ISSN: 00268925
DOI: 10.1007/PL00008652

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