Labeling of Anti-MUC-1 Binding Single Chain Fv Fragments to Surface Modified Upconversion Nanoparticles for an Initial in Vivo Molecular Imaging Proof of Principle Approach
Autor(en): | Hischemoeller, Anja Walter, Claudia Weiler, Volker Hummel, Helga Thepen, Theo Huhn, Michael Barth, Stephan Hoheisel, Werner Koehler, Karen Dimova-Landen, Diana Bremer, Christoph Haase, Markus Waldeck, Jens |
Stichwörter: | anti-MUC-1 single-chain antibody fragment; Biochemistry & Molecular Biology; CANCER; CELLS; Chemistry; Chemistry, Multidisciplinary; CYTOTOXICITY; DOPED NAYF4 NANOCRYSTALS; EMISSION; EXPRESSION; FLUORESCENCE; in vivo optical molecular imaging; labeling; NANOPHOSPHORS; QUANTUM DOTS; THERAPY; upconversion nanoparticles | Erscheinungsdatum: | 2012 | Herausgeber: | MDPI AG | Journal: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Volumen: | 13 | Ausgabe: | 4 | Startseite: | 4153 | Seitenende: | 4167 | Zusammenfassung: | In vivo optical Imaging is an inexpensive and highly sensitive modality to investigate and follow up diseases like breast cancer. However, fluorescence labels and specific tracers are still works in progress to bring this promising modality into the clinical day-to-day use. In this study an anti-MUC-1 binding single-chain antibody fragment was screened, produced and afterwards labeled with newly designed and surface modified NaYF4:Yb,Er upconversion nanoparticles as fluorescence reporter constructs. The MUC-1 binding of the conjugate was examined in vitro and in vivo using modified state-of-the-based probe to MUC-1 positive cells was clearly shown via laser scanning microscopy and in an initial proof of principal small animal optical imaging approach. |
ISSN: | 14220067 | DOI: | 10.3390/ijms13044153 |
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