Polynuclear zinc(II) complexes of thiosemicarbazone: Synthesis, X-ray structure and biological evaluation
Autor(en): | Saswati Mohanty, Monalisa Banerjee, Atanu Biswal, Sonaleen Horn, Jr., Adolfo Schenk, Gerhard Brzezinski, Krzysztof Sinn, Ekkehard Reuter, Hans Dinda, Rupam |
Stichwörter: | 3-AMINOPYRIDINE-2-CARBOXALDEHYDE THIOSEMICARBAZONE; Biochemistry & Molecular Biology; Chemistry; Chemistry, Inorganic & Nuclear; CYTOTOXIC ACTIVITIES; Cytotoxicity; DNA and HSA interaction; DNA CLEAVAGE; IN-VITRO; PHASE-II; Phosphoesterase activity; Polynuclear zinc(II) complexes; RIBONUCLEOTIDE REDUCTASE; RUTHENIUM(II) ARENE COMPLEXES; SPECTRAL CHARACTERIZATION; Thiosemicarbazone; VITRO ANTICANCER ACTIVITY; ZN(II) COMPLEXES | Erscheinungsdatum: | 2020 | Herausgeber: | ELSEVIER SCIENCE INC | Journal: | JOURNAL OF INORGANIC BIOCHEMISTRY | Volumen: | 203 | Zusammenfassung: | Two new dimeric Zn(II) ([{ZnL1(DMSO2)}(2)]center dot DMSO (1), [{(ZnLCl)-Cl-2}(2)] (2)) and a novel tetrameric Zn(II) complex ([(Zn2L3)(2)(mu-OAc)(2)(mu(3)-O)(2)] (3)), where H2L1 = 4-(p-methoxyphenyl) thiosemicarbazone of o-hydroxynapthaldehyde, HL2 = 4-(p-methoxyphenyl)thiosemicarbazone of benzoyl pyridine and H2L3 = 4-(p-chlorophenyl)thiosemicarbazone of o-vanillin are reported. Ligands and their complexes were characterized by spectroscopic and single crystal X-ray diffraction techniques. In addition, the complexes exhibited good binding affinity towards HSA (10(12)M(-1)), which is supported by their ability to quench the tryptophan fluorescence emission spectra of HSA. The complexes were also screened for their DNA binding propensity through UV-vis absorption titration, circular dichroism and fluorescence spectral studies. Results show that they effectively interact with CT-DNA through an intercalative mode of binding, with binding constants ranging from 10(3) to 10(4) M-1. Among the three complexes 1 has the highest binding affinity towards CT-DNA. Further, the phosphatase activity was evaluated using bis(2,4-dinitrophenyl)phosphate (BDNPP) as substrate, however, the complexes did not yield any measurable catalytic activity. Nevertheless the complexes showed significant cytotoxic potential against HeLa and HT-29 cancer cell lines that was assessed through MTT assay and DAPI staining. Remarkably, complex 1 showed better activity than cisplatin against HT-29 cell line. |
ISSN: | 01620134 | DOI: | 10.1016/j.jinorgbio.2019.110908 |
Zur Langanzeige
Seitenaufrufe
1
Letzte Woche
0
0
Letzter Monat
0
0
geprüft am 17.05.2024