Solid-phase synthesis of oligo(2'-deoxyxylonucleotides) and PCR amplification of base-modified DNA fragments.
| Autor(en): | Seela, F. Rosemeyer, H. Krecmerova, M. Röling, A. |
Stichwörter: | adenosine, 58-61-7; DNA, 9007-49-2; DNA directed DNA polymerase, 9012-90-2; thymidine, 50-89-5; xylose, 25990-60-7, 58-86-6; 1-(2'-deoxy-beta-threopentofuranosyl)adenine, 13276-53-4, 58-61-7; 1-(2'-deoxy-beta-threopentofuranosyl)thymine, 16053-52-4; Adenosine, 58-61-7; DNA, 9007-49-2; DNA Restriction Enzymes, EC 3.1.21.-, 58-61-7; DNA-Directed DNA Polymerase, EC 2.7.7.7; Oligonucleotides; Phosphonic Acids; Taq Polymerase, EC 2.7.7.-; Thymidine, 50-89-5, 58-61-7; Xylose; 1 (2' deoxy beta threopentofuranosyl)adenine; 1 (2' deoxy beta threopentofuranosyl)thymine; 1-(2'-deoxy-beta-threopentofuranosyl)adenine; 1-(2'-deoxy-beta-threopentofuranosyl)thymine; adenosine; DNA; DNA directed DNA polymerase; drug derivative; oligonucleotide; phosphonic acid derivative; restriction endonuclease; Taq polymerase; thymidine; xylose, article; chemical structure; chemistry; enzyme specificity; metabolism; methodology; molecular genetics; nucleotide sequence; polymerase chain reaction; restriction mapping; synthesis, Adenosine; Base Sequence; DNA; DNA Restriction Enzymes; DNA-Directed DNA Polymerase; Molecular Sequence Data; Molecular Structure; Oligonucleotides; Phosphonic Acids; Polymerase Chain Reaction; Restriction Mapping; Substrate Specificity; Taq Polymerase; Thymidine; Xylose | Erscheinungsdatum: | 1991 | Journal: | Nucleic acids symposium series | Ausgabe: | 24 | Startseite: | 87 | Seitenende: | 90 | Zusammenfassung: | 1-(2'-Deoxy-beta-D-threo-pentofuranosyl)thymine (xTd) and -adenine (xAd) were converted into their appropriately protected 3'-phosphonates 1a, 2a as well as their 2-cyanoethyl phosphoramidites 1b, 2b. These compounds were used for solid-phase syntheses of the oligo(2'-deoxy-beta-D-xylonucleotides) 5-8. Structural properties and behavior against nucleases is described. Apart from oligo(2'-deoxyxylonucleotides) the PCR-amplification of a pUC18 DNA fragment with Taq polymerase was studied in the presence of the 7-deazapurine derivatives of dGTP, dATP, and dITP. The incorporation efficiency of the modified compounds was compared with those of the parent nucleotides. 7-Deaza-2'-deoxyguanosine protected the DNA-fragment from hydrolysis by the restriction endodeoxyribonuclease Eco RI, Pst I, Bam HI, and Sma I if the nucleoside was located within the recognition site. |
ISSN: | 02613166 | Externe URL: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026289224&partnerID=40&md5=9b8b0e72c8f2dc290f59f4b300e21675 |
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